MmuEX6095872 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123401393-123407515:-
Coord C1 exon
chr4:123407438-123407515
Coord A exon
chr4:123401865-123401912
Coord C2 exon
chr4:123401393-123401563
Length
48 bp
Sequences
Splice sites
3' ss Seq
CTTTCTCTGCAATTCTCCAGCTG
3' ss Score
7
5' ss Seq
CAGGTGAAT
5' ss Score
6.6
Exon sequences
Seq C1 exon
GGAATAAAAGAGGTGCCTGGCACAATGATACATGGGAAAGTGCCTCTGGCTGCAACTGATGGATTAAATTCACATGAG
Seq A exon
CTGTTTCAGAAAGACCAAGTGGATCCTCTTCAGGTGAAATTGCAGCAG
Seq C2 exon
GTTGCACAAAGAATTGCACAACTGCAGGAGGCTCTGTTACATTGTGGGAAATTTCAAGATGCCTTAGAGCCTTTGCTCAGCTGGTTGACAGATACTGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCTGAGTATAAAGTGGTGAAAGCACAGATCCAAGAACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'117-115,'117-114,118-115
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.034 C2=0.018
Domain overlap (PFAM):
C1:
NA
A:
PF0043516=Spectrin=FE(49.1=100)
C2:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TACATGGGAAAGTGCCTCTGG
R:
TGTCAACCAGCTGAGCAAAGG
Band lengths:
140-188
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types