RnoEX6017230 @ rn6
Exon Skipping
Gene
ENSRNOG00000006731 | Spc25
Description
SPC25, NDC80 kinetochore complex component [Source:RGD Symbol;Acc:1307811]
Coordinates
chr3:55446584-55450768:-
Coord C1 exon
chr3:55450616-55450768
Coord A exon
chr3:55450446-55450511
Coord C2 exon
chr3:55446584-55446730
Length
66 bp
Sequences
Splice sites
3' ss Seq
TCTTGTAATCTGTCTTTCAGAAA
3' ss Score
9.65
5' ss Seq
ATCGTATGT
5' ss Score
5.94
Exon sequences
Seq C1 exon
CGTCTTAAAACAGAATGGGTGAAGACGAACTGGCAGCCTTCGAGAAAAGCATAAACGAATTTGGGGACAAGTTCCGGTACAGACTCAGTGATAATCGTAGCCAGGTGCTGGGACTTAAAGATGCCTTCAAGGACTCCATTAGAGCGCTTTCAG
Seq A exon
AAAAGCTGTCTTTGAAACTAAAGGAAGAAGAAAGGATGGCTGAGATGATTCTGGAATATAAAAATC
Seq C2 exon
AGCTCTGTAAGCAGAATAAGCTCATTCAAGAAAAGAAGGATAATGTGTTAAAGATGATTGCTGAAGTGAAAGGCAAAGGGCAGGAGGTGGAAGTGCTGACGGCAAACATCCAGGATCTGAAGGAGGAGTACGCCAGGAAGAAGGAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006731-'7-3,'7-2,8-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF121283=DUF3584=PU(29.5=93.6),PF083176=Spc7=PU(20.2=87.2)
A:
PF121283=DUF3584=FE(14.8=100),PF083176=Spc7=FE(10.8=100)
C2:
PF121283=DUF3584=FE(32.9=100),PF083176=Spc7=FE(24.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGCTGGGACTTAAAGATGCCT
R:
CAGATCCTGGATGTTTGCCGT
Band lengths:
168-234
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]