RnoEX6055112 @ rn6
Exon Skipping
Gene
ENSRNOG00000015751 | Cyp4f18
Description
cytochrome P450, family 4, subfamily f, polypeptide 18 [Source:RGD Symbol;Acc:1305261]
Coordinates
chr16:19501680-19507630:+
Coord C1 exon
chr16:19501680-19501878
Coord A exon
chr16:19506493-19506637
Coord C2 exon
chr16:19507577-19507630
Length
145 bp
Sequences
Splice sites
3' ss Seq
CACCGGGTCCTCTTCTACAGATC
3' ss Score
9.64
5' ss Seq
CAGGTAGAT
5' ss Score
7.25
Exon sequences
Seq C1 exon
GATGCCACTGCTGAGCCTGTCCTGGCTGGGCCTGGGGCACACAGCAGCCTCTCCATGGCTGTTGCTGCTGCTGGTTGGGGCCTCCTGTCTCCTGGCTTACATCCTGCCTCAGGTTTATGCTGTCTTTGAGAACTCTCGTCGCCTCCGCCGTTTTCCGCAGCCCCCCACACGAAACTGGCTCTTTGGACACCTAGGCCTG
Seq A exon
ATCCAGAGCTCAGAGGAAGGGCTCCTGTACATCCAGAGCCTGTCGAGAACCTTCAGGGATGTTTGCTGCTGGTGGGTGGGGCCGTGGCACCCCGTTATCCGCATATTCCACCCTGCCTTCATCAAGCCCGTGATCTTGGCTCCAG
Seq C2 exon
CTTCAGTTGCCCCCAAGGACAGAGTCTTCTACCGTTTCCTGAAGCCGTGGCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000015751-'6-8,'6-6,8-8=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=PU(3.0=21.2)
A:
PF0006717=p450=FE(10.3=100)
C2:
PF0006717=p450=FE(3.9=100)
Main Skipping Isoform:
ENSRNOT00000021609fB3828
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGCCACTGCTGAGCCTG
R:
CACGGCTTCAGGAAACGGTAG
Band lengths:
248-393
Functional annotations
There are 3 annotated functions for this event
PMID: 10409674
Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B(4) by omega-oxidation in human neutrophils. The CYP4F3 gene contains 14 exons and 13 introns. The cDNAs for CYP4F3A (the neutrophil isoform) and CYP4F3B have identical coding regions, except that they contain exons 4 (HsaEX0002240) and 3 (HsaEX0002239), respectively. Both exons code for amino acids 66-114 but share only 27% identity. When expressed in COS-7 cells, the K(m) of CYP4F3B was determined to be 26-fold higher than the K(m) of CYP4F3A using leukotriene B(4) as a substrate.
PMID: 11461919
Substrate specificity. Mutually exclusive exons, each coding for 48 amino acids, generate isoforms of human CYP4F3 that differ in substrate specificity, tissue distribution, and biological function. Isoform with HsaEX0002240 inactivates LTB4 by omega-hydroxylation (Km = 0.68 microm) but has low activity for arachidonic acid (Km = 185 microm); it is the only CYP4F isoform expressed in myeloid cells in peripheral blood and bone marrow.
PMID: 15145985
Among recombinant P450 enzymes, CYP4F2 and CYP4F3B (uses mutually exclusive exon HsaEX0002239) catalyzed mainly the omega-hydroxylation of C18-epoxides with an apparent Vmax of between 0.84 and 15.0 min_1, whereas the apparent Vmax displayed by CYP4F3A, the isoform found in leukocytes (uses mutually exclusive exon HsaEX0002240), ranged from 3.0 to 21.2 min_1.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]