RnoINT0040322 @ rn6
Intron Retention
Gene
ENSRNOG00000020579 | Col7a1
Description
collagen type VII alpha 1 chain [Source:RGD Symbol;Acc:1311417]
Coordinates
chr8:117700053-117700450:+
Coord C1 exon
chr8:117700053-117700172
Coord A exon
chr8:117700173-117700306
Coord C2 exon
chr8:117700307-117700450
Length
134 bp
Sequences
Splice sites
5' ss Seq
ATGGTAGGA
5' ss Score
6.66
3' ss Seq
ACCTCCATCCCTGATCCCAGGTC
3' ss Score
7.12
Exon sequences
Seq C1 exon
ATCCACTGGGCCCAGTGAGGAGAGTCCACTTGACTCAGGCTGGTAGCTCATCTGTCAGCATTACCTGGACTGGGGTTCCTGGCGCCACCGGATACAGGGTTTCCTGGCACTCAGGCCATG
Seq A exon
GTAGGAGGCAGGAGTTTAAGGGGGGCTATGGGAGGTGGTTCTGACTAGAATGAAGGCTAAAGATGCTTTCGGGGTCCGAGTGGAGCGCTGGGTAGTCTGACAAGTCCTGGTGTTACCTCCATCCCTGATCCCAG
Seq C2 exon
GTCCAGAGAAATCTCAATTGGTTTCTGGGGACGCCACGGTGGCTGAAATAGATGGACTGGAGCCAGACACAGAGTATATAGTGCGTGTGAGAACCCATGTAGCCGGTGTGGATGGAGCCCCGGCCTCTGTGGTTGTGAGGACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000020579:ENSRNOT00000027994:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.293 A=NA C2=0.429
Domain overlap (PFAM):
C1:
PF0004116=fn3=PU(46.8=90.2)
A:
NA
C2:
PF0004116=fn3=PD(51.9=83.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCAGTGAGGAGAGTCCACTT
R:
AGTCCTCACAACCACAGAGGC
Band lengths:
253-387
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]