RnoINT0044644 @ rn6
Intron Retention
Gene
ENSRNOG00000043513 | Cyp4x1
Description
cytochrome P450, family 4, subfamily x, polypeptide 1 [Source:RGD Symbol;Acc:628719]
Coordinates
chr5:133940261-133941027:-
Coord C1 exon
chr5:133940927-133941027
Coord A exon
chr5:133940452-133940926
Coord C2 exon
chr5:133940261-133940451
Length
475 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
3' ss Seq
GTCTTTGCTGGGTGTTACAGGCT
3' ss Score
6.88
Exon sequences
Seq C1 exon
AAAAGATTATCCAGGACAGAAAGAAAACCCTCAAGGATCAAGTAAATCAGGATGACACTCAAACATCTCAAAATTTTCTGGATATTGTTCTTTCTGCCCAG
Seq A exon
GTAAATGCACCAAAAGTCTAGGCCTGCTCAAAGAGCTGCTAACTGTGTGGGAGGGTGGGTGAGCTTGTGGAATCAGGACAGGAGGACAGAATAAATTTCCTCAGCTAAATCCTAGCAGTAGACTGCTATGAACAATTGGCTCTTGGTAGGAAAACAGAGAGCTGCTAGGAGTCAGTGAATACTAACTTACCGGTTGCCTTTGTTTGGAGGTTATGCGATACCTGGAGTTTAGAGACTCAACTCTTTAAAAGGACAAGCACTTTAAAAGGAAAAGCTGAGGGTGTCCATGCTTTTCTTATGTAACATTGGCTCTCCTTTGTGCTCTGAACAAAGTAGGACTCATAGTATGCAGTTGGTAAACTAAAATTGAAGCCTGTATTTGAAACTCTATCATTCTGTAAAGCTATCATTTTGATGGTGTCTCCAAATATCTGCGATGCACGTTTCATCCAGCAGTCTTTGCTGGGTGTTACAG
Seq C2 exon
GCTGGAGATGAAAAGGCCTTCTCAGATGCTGACCTTCGGTCTGAGGTGAACACCTTCATGTGGGCAGGACACGACGCCTCTGCAGCTAGCATCTCCTGGCTCCTTTACTGCCTGGCTCTGAACCCTGAGCATCAAGACAGATGCCGGACAGAGATCAGGAGCATTCTGGGAGATGGGTCTTCCATCACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000043513:ENSRNOT00000011985:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.088 A=NA C2=0.016
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(7.2=100)
A:
NA
C2:
PF0006717=p450=FE(13.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TATCCAGGACAGAAAGAAAACCCT
R:
TGGAAGACCCATCTCCCAGAA
Band lengths:
278-753
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]