RnoINT0070961 @ rn6
Intron Retention
Gene
ENSRNOG00000006116 | Hk2
Description
hexokinase 2 [Source:RGD Symbol;Acc:2797]
Coordinates
chr4:113569049-113570597:-
Coord C1 exon
chr4:113570293-113570597
Coord A exon
chr4:113569198-113570292
Coord C2 exon
chr4:113569049-113569197
Length
1095 bp
Sequences
Splice sites
5' ss Seq
CAGGTGCGT
5' ss Score
9.73
3' ss Seq
GTCCCGGGTTCATTTTCCAGAGA
3' ss Score
7.35
Exon sequences
Seq C1 exon
TTTTGCCAAGCGTCTCCATAAGGCAGTGAGGAGGCTGGTGCCCGACTGTGATGTCCGCTTCCTCCGCTCTGAGGATGGCAGCGGCAAGGGGGCTGCTATGGTGACGGCGGTGGCTTACCGTCTGGCTGACCAACACCGGGCCCGCCAGAAGACCCTGGAGTCTCTGAAGCTGAGCCACGAGCAGCTTCTGGAGGTTAAGAGAAGAATGAAGGTGGAAATGGAGCAGGGTCTGAGCAAGGAGACGCATGCGGTCGCCCCTGTGAAGATGCTGCCCACTTACGTGTGTGCCACTCCAGATGGCACAG
Seq A exon
GTGCGTGGCATCATCGGCACTGCGCGTGCGGGTCTGTGTGTGCGCGTGTGTGCATGCATGTCTGTGTGCGTGTCTGTGTGTGTGTGTCTGTGTGCATGTATGCATGCGGGTCTGTGTGCGTGTCTGTGTGTGTGTGTCTGTGTGCGTGTGTCTGTGCGTGTGTGCATGTGTGTCTGTGCGTGTGTGTGAGTGTGAGTGAGTTGTGCGTCGTGTGCCAAGTGGTATCCGGTCCCCCTGGAGCTTACCTGTCAGTTGGGAAACCCCTGATGTGGGTATTAATTAGGAACTGAACTTGGGACCACTGCAAGAACAATGTCAGCTCTAAACTGCCAAATTATCTCTTCAGCCCCATTTGTTTTGCTTTTTGAAATGGGTCTGATGTAGCTGAGGCCAGCCTCAAATGTAGTCCAGGATGTTGTAGAGGACAACCTTGAATTACCTATCCTAGGATTGTACCTATATGCAGAGACACTCAGAGACCAATTTTGTTTATTAAAAATTTGCTGTTTGGCAGAGAACTAAAACCAAGAGAACCCTGTTCACGGTCACATAATCAGTGTCAGCCTCCATTCAAACCTAGCATGCGAGAGGCAGTTCTCAAATACTACATTAGTTTGGGTCTGCATGTATTTCCTGTTTGGCCCCATAGAAAACGTTTCTGGTTCATGATGGTTCATGGTTGGCATAGGCTCCAGAGGCAGAAGCTAGGGTTTGGTACCCTGGTAATGTGGGGCCTTACTCCATTCAAGTACCCCTCAAAGATCACCTTTAAGAGGGAAAAGGAATGTGCTGGATTGGCTCATGGTTGATTTAAACTCAAAATGTAGCCATTCGTCTCGTCTCAGCTGTGACATTTAGACAGTCTTAGGTATACCGTATTAGCATTCCCCATGTTCTGAAGAGCTGACTGCTGGGGTTCTACCACATGGGCCCACTCGGATTTGGTCTTGAAGATTTTGCTTGCTTAACTGCCAGGTGGCGCTCACGCTTCATCTTCTCCCAAGACCTGCGTTTCACAGGGTCTCTTCCACAACCCCAAGGAAGGGAAACGCATTCTCTGTCACCCCAACTCATTGTCCCGGGTTCATTTTCCAG
Seq C2 exon
AGAAAGGAGACTTCTTGGCCTTGGATCTTGGAGGAACAAACTTCCGGGTCCTGCTGGTGCGTGTGCGTAATGGCAAGCGGAGGGGCGTGGAGATGCATAACAAGATCTACTCCATCCCACAGGAGGTTATGCATGGCACTGGGGAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006116:ENSRNOT00000008813:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.010 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=PD(16.7=38.8),PF0034916=Hexokinase_1=PU(29.1=58.3)
A:
NA
C2:
PF0034916=Hexokinase_1=FE(23.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAGCGTCTCCATAAGGCAG
R:
GCAGGACCCGGAAGTTTGTTC
Band lengths:
354-1449
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]