RnoINT0096473 @ rn6
Intron Retention
Gene
ENSRNOG00000046276 | Myh3
Description
myosin heavy chain 3 [Source:RGD Symbol;Acc:3138]
Coordinates
chr10:53634298-53634587:+
Coord C1 exon
chr10:53634298-53634385
Coord A exon
chr10:53634386-53634469
Coord C2 exon
chr10:53634470-53634587
Length
84 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
3' ss Seq
TATCCTGCCTGTGTTCTTAGGGG
3' ss Score
9.46
Exon sequences
Seq C1 exon
GAAAACTTGAACAAGCTGATGTCCAATTTAAGAACCACTCACCCACACTTTGTGCGCTGTATAATACCCAATGAAACCAAGACCCCAG
Seq A exon
GTAAGACGCCTGCCAGCCCTGAGGTTACCGCTCAGTCTTTAAGGACCTGTGGGTGAAGCTCGGGTATCCTGCCTGTGTTCTTAG
Seq C2 exon
GGGCCATGGAACACAGCCTGGTCCTGCACCAGCTGAGGTGTAACGGGGTGCTAGAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAACAGGATTCTGTATGGAGACTTTAAACAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000046276:ENSRNOT00000004147:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.3=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTTGAACAAGCTGATGTCCA
R:
GAACCCCTTCCTGCAGATGC
Band lengths:
167-251
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]