RnoINT0123026 @ rn6
Intron Retention
Gene
ENSRNOG00000018901 | Rab14
Description
RAB14, member RAS oncogene family [Source:RGD Symbol;Acc:620881]
Coordinates
chr3:14384448-14386118:-
Coord C1 exon
chr3:14385472-14386118
Coord A exon
chr3:14384502-14385471
Coord C2 exon
chr3:14384448-14384501
Length
970 bp
Sequences
Splice sites
5' ss Seq
TTGGTAAGT
5' ss Score
10.47
3' ss Seq
CGTCTGCTTTCTTTACACAGGGG
3' ss Score
9.42
Exon sequences
Seq C1 exon
ATCACAACTTACTGATCAGTTAACAAGGAAAAGAACCTTAAAGGGAGAAGGAGCTATCTAATGATGTTTGACTTAAATTTATAAAAATTTTCTTCTTTGAAAGATTTCCAGAATAACTTTTTGTTGATATTACTCCTTTTTTCGTATATTTCCTCGAGTAAAAATTTGGAGTGTTTTGAAACAATTTTTTCATATTTTTGTGGATCACTTGAGAATATTAAAGTAACATTCAAGAGAGTTGTGAGGGTTGGGGATTTAGCTCAGTGGTAGAGCACTTGCCTAGCAAGCGCAAGGCCCTGGGTTCGGTCCCCAGCTCCAAAAAAAAGAAAAACAACAACAACAAAAAAACAAAAAAAAAAAACCAAAAAAAAAAGAGAGTTTGTGATCAGGTTGGGAATTTTACTGACTTAGATCAAAATTTTGTTTGTATAGTCAGTGTGTTTTCGTAATATGTATGATATTTTTAAATGATGTGTATGTATTTGTGTGTGTGTGTGTGTGTGTGTATGTGTTTGGAAAGGGCGGCAGTCATAGGTTTCTATAACTTGTGAACGTTTGCTGAATTAACATAGTTTTCTTTGTTTTCAGTGCCACCATGGCAACTGCACCGTACAACTACTCTTACATCTTTAAGTATATTATTATTG
Seq A exon
GTAAGTTTACTTAACGTGTATGCTTGTCCTCCAAAGAAAGCTAACACATGGAACATTTATACATCAGTACCTGAACATACTACTGCAGACGTGCATGCTTTTGATGACTTCAGAACTAAAGCTTTTTGATGAAGACACACATGCCTTTTCCATCATGGCCCCGGGTAGTGAAATGATGGTGGTTGTTCTTCAGGGCCACCTGCGAACATGTTAGTGTTGGGGTTATAAAATTAATTTCCTGCTTGTGCTGAAATATCCCAATGTCCTAAAAGTTTCAGGTCCTCTTGAATTCCAAAATAGAGAGAGAGCACCGATTCAGAATTGGTCGTGAAACACACACACACACACACACACACACACACACACACACACACACAGAGTTTTGTTTCTTTTTAAATGTCAAGACCTTGCTCAGATTGTCTTAATGCATTTATCCATGAGTGGTTGTAAATAACTGGAAAAGCCCAGATGGTAGGCTGAGTTGCAGACCAGTAGAAAAGCTCTTACAAGCTTATCCTGGCTGTGGACTTGTGTTTGATCTCCACAATGGAAAATAAAACACAACCTGTAAATAAGAAAAGACAAGAAAGGCCATGTCAAGTTTGTATAGCTTTGTGAGATGGCATCTACTGTAATGTAGAGATAAAACGAGACATAGTGAGCCAAGCTTCCAATTTTTAATTTGTTATTTATTTCCGTTTGCTTTTGTGATAGTTCGTTTGGATAGGCCAATGAATGCACTTGGGCTACCCATTTGGAAACAGTTCAGATTACAAGTACTATTCACCTTGTTGGTTGTTTACTAGTCTATCCTGCTAAAGTTAGAAGTGCTCAGTTTGCCTTTTGAGGAGGAAGATGCAGGGGCTGCAGTGTGATGTGCATGATGTTCTGTTACGCAGCAGTTATTGCATACAGTGATGCTGTGCATGAAGGCTGAGAAGTATAACTGCCGTCTGCTTTCTTTACACAG
Seq C2 exon
GGGATATGGGAGTAGGAAAATCTTGCTTGCTTCATCAATTTACAGAAAAAAAGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000018901:ENSRNOT00000025649:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0007117=Ras=PU(3.1=27.8)
A:
NA
C2:
PF0007117=Ras=FE(11.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCGGTCCCCAGCTCCAAAAA
R:
AAGCAAGATTTTCCTACTCCCA
Band lengths:
374-1344
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]