BtaEX0015092 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000008926 | FUBP1
Description
Bos taurus far upstream element (FUSE) binding protein 1 (FUBP1), mRNA. [Source:RefSeq mRNA;Acc:NM_001076846]
Coordinates
chr3:66969542-66971388:+
Coord C1 exon
chr3:66969542-66969635
Coord A exon
chr3:66970467-66970529
Coord C2 exon
chr3:66971350-66971388
Length
63 bp
Sequences
Splice sites
3' ss Seq
TCTTTTTATTTTATGTGTAGATG
3' ss Score
7.62
5' ss Seq
AAGGCAGGA
5' ss Score
1.7
Exon sequences
Seq C1 exon
AAGATTGCAGCAAAAATTGGAGGTGATGCTGGTACGTCACTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCTTTGGAAGATGGAG
Seq A exon
ATGGCTCTTGGACAAGTCCGAGCAGTACAACACACTGGGAGGGAATGCCCTCTCCTTTTAAAG
Seq C2 exon
ATCAGCCAGATGCTAAGAAAGTTGCTCCTCAAAATGACT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000008926_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.865 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSBTAT00000011751fB2874
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGATTGCAGCAAAAATTGGAGGTG
R:
AGTCATTTTGAGGAGCAACTTTCT
Band lengths:
132-195
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.748, FDR=0.000), PC9 Depleted at 14 days (FC=0.663, FDR=0.000), Late Xenograft No change (FC=0.803, FDR=0.015).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development