MmuEX0019729 @ mm10
Exon Skipping
Gene
ENSMUSG00000028034 | Fubp1
Description
far upstream element (FUSE) binding protein 1 [Source:MGI Symbol;Acc:MGI:1196294]
Coordinates
chr3:152213977-152215630:+
Coord C1 exon
chr3:152213977-152214067
Coord A exon
chr3:152214825-152214887
Coord C2 exon
chr3:152215592-152215630
Length
63 bp
Sequences
Splice sites
3' ss Seq
TCTTTTTATTTTATGTGTAGATG
3' ss Score
7.62
5' ss Seq
AAGGCAGGA
5' ss Score
1.7
Exon sequences
Seq C1 exon
ATTGCAGCAAAAATTGGGGGTGATGCTGGTACATCATTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCCTTAGAAGATGGAG
Seq A exon
ATGGCTCTTGGACAAATCCGAGCAGTACCACACACTGGGAGGGAATGCCCTCTCCTTTTAAAG
Seq C2 exon
ATCAGCCAGATGCTAAGAAAGTACCTCCCCAAAATGACT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028034_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.933 A=1.000 C2=0.983
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGCAGCAAAAATTGGGGGTG
R:
TTTTGGGGAGGTACTTTCTTAGCA
Band lengths:
124-187
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.748, FDR=0.000), PC9 Depleted at 14 days (FC=0.663, FDR=0.000), Late Xenograft No change (FC=0.803, FDR=0.015).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types