RnoEX0037091 @ rn6
Exon Skipping
Gene
ENSRNOG00000043370 | Fubp1
Description
far upstream element binding protein 1 [Source:RGD Symbol;Acc:1591892]
Coordinates
chr2:257429555-257431237:+
Coord C1 exon
chr2:257429555-257429645
Coord A exon
chr2:257430395-257430457
Coord C2 exon
chr2:257431199-257431237
Length
63 bp
Sequences
Splice sites
3' ss Seq
TCTTTTTATTTTATGTGTAGATG
3' ss Score
7.62
5' ss Seq
AAGGCAGGA
5' ss Score
1.7
Exon sequences
Seq C1 exon
ATTGCAGCAAAAATTGGGGGTGATGCTGGTACATCATTGAATTCAAATGACTATGGTTATGGGGGACAAAAGAGACCCTTAGAAGATGGAG
Seq A exon
ATGGCTCTTGGACAAATCCGAGCAGTACCACACACTGGGAGGGAATGCCCTCTCCTTTTAAAG
Seq C2 exon
ATCAACCAGATGCTAAGAAAGTACCTCCCCAAAATGATT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000043370_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.871 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSRNOT00000066199fB5748
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGCAGCAAAAATTGGGGGTG
R:
TTTTGGGGAGGTACTTTCTTAGCA
Band lengths:
124-187
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.748, FDR=0.000), PC9 Depleted at 14 days (FC=0.663, FDR=0.000), Late Xenograft No change (FC=0.803, FDR=0.015).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]