BtaEX0021018 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107228118-107230273:-
Coord C1 exon
chr3:107230114-107230273
Coord A exon
chr3:107229433-107229759
Coord C2 exon
chr3:107228118-107228202
Length
327 bp
Sequences
Splice sites
3' ss Seq
TTTTCCATCTGGATTTCCAGGCT
3' ss Score
7.09
5' ss Seq
CTGGTATTC
5' ss Score
1.5
Exon sequences
Seq C1 exon
GCAGAAACAGCTGGAAGATATCCTGGTTCTGGCCAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTCGCCAACTCAGAACCTGTTGGCACCCAGACTGCTAAAATACAGCAGCAGATCATTCGGCACAAG
Seq A exon
GCTCTGGAGGAGGAAATAGAAAGCCATGCAGCAGATGTGCACCAGGCAGTCACAGTTGGGCAGTCCCTCTCCTCCCTGACAAGTCCCGCAGAGCAGGGTGTGCTGTCAGAAAGGCTAGACTCGTTGCAGGCCCGATTCAGTGAGATTCAAGACCGGTGCGGCCGGAAAGCAGCCCTGCTTGAACAAGCACTGTCTAATGCTAGGCTTTTCGGGGAGGATGAGGTGGAGGTGCTCAACTGGCTGGCTGAGGTTGAGGACAAGCTCAGTTCAGTGTTCGTAAAGGATTACAGACAGGATGTCCTGGAGAAGCAGCATGCTGACCACCTG
Seq C2 exon
GCTTTAAATGAAGAAATTGTTAATAGAAAGAAGAATGTAGATCAAGCTATTAAAAACGGTCAGGCTCTTCTGAAACAAACCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024_CASSETTE5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.109 A=0.000 C2=0.030
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
A:
PF0043516=Spectrin=PD(57.0=56.0),PF0043516=Spectrin=PU(41.1=40.4)
C2:
PF0043516=Spectrin=FE(26.2=100)
Main Inclusion Isoform:
ENSBTAT00000033270fB4009
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGAAACAGCTGGAAGATATC
R:
TTGTTTCAGAAGAGCCTGACCG
Band lengths:
238-565
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development