Special

MmuEX0027472 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123073156-123075150:-
Coord C1 exon
chr4:123074991-123075150
Coord A exon
chr4:123074337-123074663
Coord C2 exon
chr4:123073156-123073240
Length
327 bp
Sequences
Splice sites
3' ss Seq
TTTTCCATCTGGATTTCCAGGCT
3' ss Score
7.09
5' ss Seq
CTGGTATTT
5' ss Score
3.5
Exon sequences
Seq C1 exon
GCAAAAACAGCTGGAAGATATCTTGGTTCTGGCTAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTTGCTAACTCGGAACCTGTTGGCACTCAGACTGCCAAAATACACCAGCAGATTATTCGTCACAAG
Seq A exon
GCTCTAGAGGAAGAAATAGAAAACCATGCAACAGATGTGCACCAGGCAGTTAAAATTGGGCAGTCCCTCTCCTCCCTGACATGTCCGGCAGAGCAGGGCATCATGTCAGAAAAGCTAGACTCATTGCAGGCCCGATACAGTGAAATTCAAGACCGGTGCTGTCGGAAAGCATCCCTGCTCGAGCAGGCCCTGTTCAATGCTAGGCTGTTCGGGGAGGATGAGGTGGAGGTGCTCAACTGGCTGGCTGAGGTTGAGGACAAGCTCAGTACAGTGTTCGTAAAGGATTACAGACAGGATGTCCTGCAGAAACAGCATGCCGACCACCTG
Seq C2 exon
GCTTTAAATGAAGAGATTATTAATAGAAAGAAGAATGTAGATCAAGCAATAAAAAATGGTCAGGCTCTTCTAAAACAAACTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_CASSETTE5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (No Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.102 A=0.009 C2=0.078
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)

							
A:
PF0043516=Spectrin=PD(57.0=56.0),PF0043516=Spectrin=PU(40.6=39.4)

							
C2:
PF0043516=Spectrin=FE(26.2=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000101819





     
                   









    
Main Skipping Isoform:
ENSMUSP00000081324f2416A





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000095509, ENSMUSP00000114568
          









    
Other Skipping Isoforms:
ENSMUSP00000080755, ENSMUSP00000081324, ENSMUSP00000081324f6186A, ENSMUSP00000095508, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000101831f2417A, ENSMUSP00000119885
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39427961-39428287 
ALTERNATIVE
HsaEX0037154
(MACF1)
Human
(hg19)
chr1:39893633-39893959 
ALTERNATIVE
HsaEX0037154
(MACF1)
Rat
(rn6)
chr5:141086342-141086668 
ALTERNATIVE
RnoEX0051771
(Macf1)
Cow
(bosTau6)
chr3:107229433-107229759 
ALTERNATIVE
BtaEX0021018
(MACF1)
Chicken
(galGal4)
chr23:5377439-5377765 
ALTERNATIVE
GgaEX0004831
([1])
Chicken
(galGal3)
chr23:5694786-5695112 
ALTERNATIVE
GgaEX0004831
(MACF1)
Zebrafish
(danRer10)
chr19:35495015-35495341 
ALTERNATIVE
DreEX0044255
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCAAAAACAGCTGGAAGATATCTTGG

				
R: 
AGTTTGTTTTAGAAGAGCCTGACCA

				
Band lengths: 
241-568

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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