MmuEX6095875 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123072249-123075150:-
Coord C1 exon
chr4:123074991-123075150
Coord A exon
chr4:123073156-123073240
Coord C2 exon
chr4:123072249-123072490
Length
85 bp
Sequences
Splice sites
3' ss Seq
ATGCTCTTCTTTCCTTTCAGGCT
3' ss Score
11.34
5' ss Seq
CAGGTACTT
5' ss Score
8.17
Exon sequences
Seq C1 exon
GCAAAAACAGCTGGAAGATATCTTGGTTCTGGCTAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTTGCTAACTCGGAACCTGTTGGCACTCAGACTGCCAAAATACACCAGCAGATTATTCGTCACAAG
Seq A exon
GCTTTAAATGAAGAGATTATTAATAGAAAGAAGAATGTAGATCAAGCAATAAAAAATGGTCAGGCTCTTCTAAAACAAACTACAG
Seq C2 exon
GCGAGGAGGTACTGCTTATCCAGGAGAAGCTGGATGGGATAAAGACCCGCTATGCGGACATCACTCTCACTAGCTCCAAGGCCCTCAGAACTTTAGAACAAGCCCGGCAGCTGGCCACCAAGTTCCACTCTACATATGAGGAACTAACTGGGTGGCTGAGGGAGGCGGAGGAGGAGCTGGCAGCCAGTGGAGGACAGTCTCCCACAGGGGAGCAAATACCCCAGTTTCAGCAAAGACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'82-81,'82-80,85-81=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.102 A=0.078 C2=0.296
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
A:
PF0043516=Spectrin=FE(26.2=100)
C2:
PF0043516=Spectrin=PD(30.8=40.7),PF0043516=Spectrin=PU(41.5=54.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCCATGAGACAGCTGAGCCT
R:
AGCGGGTCTTTATCCCATCCA
Band lengths:
171-256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: