DreEX6073724 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35492025-35495341:-
Coord C1 exon
chr19:35495015-35495341
Coord A exon
chr19:35492346-35492430
Coord C2 exon
chr19:35492025-35492251
Length
85 bp
Sequences
Splice sites
3' ss Seq
CTACTACTGGTCCCCTACAGGCT
3' ss Score
7.9
5' ss Seq
CTGGTACTT
5' ss Score
4.74
Exon sequences
Seq C1 exon
GCTCTCCAAGAGGAAGTCTCCTCTCGAGAGAATAATGTAGACCGTCTTGAGAGTCTCAGCCAATCGCTCCATCCGCTCAGCTGCGCAGCCGATCAGGATTGGCTGGGCGAGCGTGTGGGTGCAGTGCGCAGCGGGCACACAGAGCTGCGTGATTGGTGCGTTCGGAGAGAGGCCATGCTGGAGCAGGCGCTGGCCAATGCACAGCTGTTTGGAGAGGAAGAGGTGGAGGTCCTGAACTGGCTAGCTGAGGTGGCCCAGAGACTGGCTGAGGTCTCAGTGCAGAGTTATCAGCCGGAGCACCTAGAGCAGCAGCACAAACACACACTG
Seq A exon
GCTCTTAATGAAGAGATTGTCAGCAGGAAGAAGACTGTGGACCAGGCCATTAAGAACGGACAGGCTCTGCTCAAACAAACCACTG
Seq C2 exon
GCGAGGAGGTCTTGTTGATCCAAGAGAAGTTGGATGGCATCAAAAGTCGCTACGCTGAAATGACGGCGGGCAGTAGCAAGGCCCTTCGGACCCTGGAACAGGCCCTTCAGTTGGCCACACGATTTGCCAGCTCACACGATGACATCAATCAATGGCTGGATAGAGTGGAGGCGGAGCTTAACAACGCTGAGCCAGACACAACACCAGCCTATCAGGACAGACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'76-80,'76-79,77-80=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.147 A=0.034 C2=0.289
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(57.0=56.0),PF0043516=Spectrin=PU(41.1=40.4)
A:
PF0043516=Spectrin=FE(26.2=100)
C2:
PF0043516=Spectrin=PD(30.8=43.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTGCAGAGTTATCAGCCGG
R:
AATCGTGTGGCCAACTGAAGG
Band lengths:
177-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]