MmuEX6095875 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123391051-123401563:-
Coord C1 exon
chr4:123401393-123401563
Coord A exon
chr4:123395913-123395997
Coord C2 exon
chr4:123391051-123391170
Length
85 bp
Sequences
Splice sites
3' ss Seq
ATGCTCTTCTTTCCTTTCAGGCT
3' ss Score
11.34
5' ss Seq
CAGGTACTT
5' ss Score
8.17
Exon sequences
Seq C1 exon
GTTGCACAAAGAATTGCACAACTGCAGGAGGCTCTGTTACATTGTGGGAAATTTCAAGATGCCTTAGAGCCTTTGCTCAGCTGGTTGACAGATACTGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCTGAGTATAAAGTGGTGAAAGCACAGATCCAAGAACAGAAG
Seq A exon
GCTTTAAATGAAGAGATTATTAATAGAAAGAAGAATGTAGATCAAGCAATAAAAAATGGTCAGGCTCTTCTAAAACAAACTACAG
Seq C2 exon
TACGAGCAGGCTGCTGATGCAGAACTAGCTTGGGTTGCTGAAACCAAGCGGAAGCTGATGGCTCTCGGTCCAATTCGTCTAGAACAAGACCAGACTACTGCTCAGCTGCAGGTGCAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_MULTIEX2-4/6=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.018 A=0.088 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
A:
PF0043516=Spectrin=FE(26.2=100)
C2:
PF0043516=Spectrin=FE(36.4=100)
Main Skipping Isoform:
ENSMUST00000084301fB12435
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTTTGCTCAGCTGGTTGACA
R:
CGAATTGGACCGAGAGCCATC
Band lengths:
179-264
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types