BtaEX6018689 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107227383-107230273:-
Coord C1 exon
chr3:107230114-107230273
Coord A exon
chr3:107228118-107228202
Coord C2 exon
chr3:107227383-107227624
Length
85 bp
Sequences
Splice sites
3' ss Seq
ATGGTGTTCTTTCCTTTCAGGCT
3' ss Score
11.12
5' ss Seq
CAGGTACTT
5' ss Score
8.17
Exon sequences
Seq C1 exon
GCAGAAACAGCTGGAAGATATCCTGGTTCTGGCCAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTCGCCAACTCAGAACCTGTTGGCACCCAGACTGCTAAAATACAGCAGCAGATCATTCGGCACAAG
Seq A exon
GCTTTAAATGAAGAAATTGTTAATAGAAAGAAGAATGTAGATCAAGCTATTAAAAACGGTCAGGCTCTTCTGAAACAAACCACAG
Seq C2 exon
GTGAAGAGGTATTGCTCATTCAAGAGAAGCTGGATGGCATAAAGACTCGCTATGCAGACATCACAGTCACTAGCTCCAAGGCTCTCAGAACCTTAGAGCAAGCCCGGCAGCTGGCCACCAAGTTCCAGTCCACATATGAGGAACTGACCGGGTGGCTGAGGGAGGTAGAGGAGGAGCTGGCAGCCAGCGGAGGACAGTCCCCCACAGGGGAACAAATACCCCAGTTTCAGCAGAGACAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'75-77,'75-76,77-77=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.109 A=0.030 C2=0.272
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
A:
PF0043516=Spectrin=FE(26.2=100)
C2:
PF0043516=Spectrin=PD(30.8=40.7),PF0043516=Spectrin=PU(41.1=54.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGCTCGCCAACTCAGAACC
R:
CGGGCTTGCTCTAAGGTTCTG
Band lengths:
176-261
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]