MmuEX6095876 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123071313-123073240:-
Coord C1 exon
chr4:123073156-123073240
Coord A exon
chr4:123072249-123072490
Coord C2 exon
chr4:123071313-123071519
Length
242 bp
Sequences
Splice sites
3' ss Seq
GTGGCTGCTGTTCTTCACAGGCG
3' ss Score
8.24
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
Exon sequences
Seq C1 exon
GCTTTAAATGAAGAGATTATTAATAGAAAGAAGAATGTAGATCAAGCAATAAAAAATGGTCAGGCTCTTCTAAAACAAACTACAG
Seq A exon
GCGAGGAGGTACTGCTTATCCAGGAGAAGCTGGATGGGATAAAGACCCGCTATGCGGACATCACTCTCACTAGCTCCAAGGCCCTCAGAACTTTAGAACAAGCCCGGCAGCTGGCCACCAAGTTCCACTCTACATATGAGGAACTAACTGGGTGGCTGAGGGAGGCGGAGGAGGAGCTGGCAGCCAGTGGAGGACAGTCTCCCACAGGGGAGCAAATACCCCAGTTTCAGCAAAGACAGAAG
Seq C2 exon
GAACTGAAGAAGGAGGTCATGGAACACAGGCTGGTGTTGGACACAGTGAATGAGGTGAGCCATGCTCTCCTAGAGCTGGTGCCTTGGAGAGCCAGAGAAGGACTGGATAAACTTGTGTCTGATGCCAACGAGCAGTACAAACTGATCAGTGACACTGTTGGACAAAGGGTAGATGAAATTGATGCTGCTATTCAGAGATCACAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'85-82,'85-81,86-82=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.078 A=0.296 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(26.2=100)
A:
PF0043516=Spectrin=PD(30.8=40.7),PF0043516=Spectrin=PU(41.5=54.3)
C2:
PF0043516=Spectrin=PD(56.6=87.0),PF0043516=Spectrin=PU(3.7=5.8)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAAAGAAGAATGTAGATCAAGCAAT
R:
CTGTTGTGATCTCTGAATAGCAGCA
Band lengths:
268-510
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: