DreEX6073725 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35491350-35492430:-
Coord C1 exon
chr19:35492346-35492430
Coord A exon
chr19:35492025-35492251
Coord C2 exon
chr19:35491350-35491556
Length
227 bp
Sequences
Splice sites
3' ss Seq
TGTCTGTCTGTCTGTGACAGGCG
3' ss Score
9.92
5' ss Seq
AAGGTGAAG
5' ss Score
5.73
Exon sequences
Seq C1 exon
GCTCTTAATGAAGAGATTGTCAGCAGGAAGAAGACTGTGGACCAGGCCATTAAGAACGGACAGGCTCTGCTCAAACAAACCACTG
Seq A exon
GCGAGGAGGTCTTGTTGATCCAAGAGAAGTTGGATGGCATCAAAAGTCGCTACGCTGAAATGACGGCGGGCAGTAGCAAGGCCCTTCGGACCCTGGAACAGGCCCTTCAGTTGGCCACACGATTTGCCAGCTCACACGATGACATCAATCAATGGCTGGATAGAGTGGAGGCGGAGCTTAACAACGCTGAGCCAGACACAACACCAGCCTATCAGGACAGACAGAAG
Seq C2 exon
GAGCTGAAGTGTGTTTCTGCTGAGAAGAGGTTAGTTCTGGACACAGTGAATGAGGTGGGCAGTGCTTTACTGGATCTGGTACCCTGGCGTGCACGGGAAGGACTGGACCGCCTGGTTGCTGATGCCAATCAGCGATACCGCCAAGCAGACGAGACCATCACACAGAGAGTGCAGTTAGTGCAGGCTGCCATCCAAAGGTCACAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'77-81,'77-80,78-81=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.034 A=0.289 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(26.2=100)
A:
PF0043516=Spectrin=PD(30.8=43.4)
C2:
PF0043516=Spectrin=PU(3.7=5.8)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAAGAGATTGTCAGCAGGAAGA
R:
CTGCTGTGACCTTTGGATGGC
Band lengths:
284-511
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]