RnoEX0051757 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141083317-141085387:-
Coord C1 exon
chr5:141085303-141085387
Coord A exon
chr5:141084398-141084639
Coord C2 exon
chr5:141083317-141083523
Length
242 bp
Sequences
Splice sites
3' ss Seq
ATGCTCCTTCTTCTTCACAGGTG
3' ss Score
10.59
5' ss Seq
AAGGTATGC
5' ss Score
9.55
Exon sequences
Seq C1 exon
GCTTTAAATGAAGAGATTATTAATAGAAAGAAGAATGTAGATCAAGCTATTAAAAATGGTCAGGCTCTTCTAAAACAAACTACAG
Seq A exon
GTGAGGAGGTGTTACTTATCCAGGAGAAGCTGGATGGAATAAAGACCCGTTATGCAGACATCACTGTCACTAGCTCCAAGGCCCTCAGAACTTTAGAACAAGCCCGGCAGCTGGCCACCAAGTTCCATTCTACATATGAGGAACTGACTGGGTGGCTGAGGGAGGTGGAGGAGGAGCTGGCGGCCAGTGGAGGACAGTCTCCCACAGGGGAGCAGATACCCCAGTTTCAGCAAAGGCAGAAG
Seq C2 exon
GAACTGAAGAAGGAGGTCATGGAGCACAGGCTGGTGTTGGACACCGTGAATGAGGTGAGCCATGCGCTCCTAGAGCTGGTGCCTTGGAGAGCCAGAGAAGGACTGGATAAACTTGTGTCTGATGCCAACGAGCAGTACAAACTGGTCAGTGACACCGTTGGACAAAGGGTAGATGAAATTGATGCTGCTATTCAGAGATCACAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'96-104,'96-103,97-104
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.023 A=0.272 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(26.4=100)
A:
PF0043516=Spectrin=PD(31.1=40.7),PF0043516=Spectrin=PU(41.1=54.3)
C2:
PF0043516=Spectrin=PD(57.0=88.4),PF0043516=Spectrin=PU(3.7=5.8)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATCAAGCTATTAAAAATGGTCAGGC
R:
TGTTGTGATCTCTGAATAGCAGCA
Band lengths:
252-494
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]