BtaEX6018690 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107226506-107228202:-
Coord C1 exon
chr3:107228118-107228202
Coord A exon
chr3:107227383-107227624
Coord C2 exon
chr3:107226506-107226712
Length
242 bp
Sequences
Splice sites
3' ss Seq
CTGTGTGCTGTCTCCCACAGGTG
3' ss Score
13.99
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GCTTTAAATGAAGAAATTGTTAATAGAAAGAAGAATGTAGATCAAGCTATTAAAAACGGTCAGGCTCTTCTGAAACAAACCACAG
Seq A exon
GTGAAGAGGTATTGCTCATTCAAGAGAAGCTGGATGGCATAAAGACTCGCTATGCAGACATCACAGTCACTAGCTCCAAGGCTCTCAGAACCTTAGAGCAAGCCCGGCAGCTGGCCACCAAGTTCCAGTCCACATATGAGGAACTGACCGGGTGGCTGAGGGAGGTAGAGGAGGAGCTGGCAGCCAGCGGAGGACAGTCCCCCACAGGGGAACAAATACCCCAGTTTCAGCAGAGACAGAAG
Seq C2 exon
GAACTAAAGAAGGAGGTCATGGAGCACAGGCTGGTGTTGGACACAGTGAATGAGGTGAGCCGTGCTCTCTTAGAGCTGGTGCCCTGGAGAGCCAGAGAAGGGCTGGATAAACTCGTGTCCGACGCCAACGAACAGTACAAGCTGGTCAGTGACACTGTTGGACAAAGGGTGGATGAAATTGATGCTGCTATTCAGAGATCACAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'77-78,'77-77,78-78=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.030 A=0.272 C2=0.000
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(26.2=100)
A:
PF0043516=Spectrin=PD(30.8=40.7),PF0043516=Spectrin=PU(41.1=54.3)
C2:
PF0043516=Spectrin=PD(57.0=88.4),PF0043516=Spectrin=PU(3.7=5.8)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATCAAGCTATTAAAAACGGTCAGGC
R:
TGTTGTGATCTCTGAATAGCAGCA
Band lengths:
252-494
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]