Special

MmuEX0027464 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123068294-123072490:-
Coord C1 exon
chr4:123072249-123072490
Coord A exon
chr4:123071313-123071519
Coord C2 exon
chr4:123068294-123068413
Length
207 bp
Sequences
Splice sites
3' ss Seq
GAAAACCTTTCCCTCCTTAGGAA
3' ss Score
9.53
5' ss Seq
CAGGTAATG
5' ss Score
9.43
Exon sequences
Seq C1 exon
GCGAGGAGGTACTGCTTATCCAGGAGAAGCTGGATGGGATAAAGACCCGCTATGCGGACATCACTCTCACTAGCTCCAAGGCCCTCAGAACTTTAGAACAAGCCCGGCAGCTGGCCACCAAGTTCCACTCTACATATGAGGAACTAACTGGGTGGCTGAGGGAGGCGGAGGAGGAGCTGGCAGCCAGTGGAGGACAGTCTCCCACAGGGGAGCAAATACCCCAGTTTCAGCAAAGACAGAAG
Seq A exon
GAACTGAAGAAGGAGGTCATGGAACACAGGCTGGTGTTGGACACAGTGAATGAGGTGAGCCATGCTCTCCTAGAGCTGGTGCCTTGGAGAGCCAGAGAAGGACTGGATAAACTTGTGTCTGATGCCAACGAGCAGTACAAACTGATCAGTGACACTGTTGGACAAAGGGTAGATGAAATTGATGCTGCTATTCAGAGATCACAACAG
Seq C2 exon
TACGAGCAGGCTGCTGATGCAGAACTAGCTTGGGTTGCTGAAACCAAGCGGAAGCTGATGGCTCTCGGTCCAATTCGTCTAGAACAAGACCAGACTACTGCTCAGCTGCAGGTGCAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'86-83,'86-82,87-83
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.296 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0043516=Spectrin=PD(30.8=40.7),PF0043516=Spectrin=PU(41.5=54.3)

							
A:
PF0043516=Spectrin=PD(56.6=87.0),PF0043516=Spectrin=PU(3.7=5.8)

							
C2:
PF0043516=Spectrin=FE(36.4=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000081324f2416A





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000080755, ENSMUSP00000081324, ENSMUSP00000081324f6186A, ENSMUSP00000095508, ENSMUSP00000095509, ENSMUSP00000101819, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000101831f2417A, ENSMUSP00000114568, ENSMUSP00000119885
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39430702-39430908 
HIGH PSI
HsaEX0037156
(MACF1)
Human
(hg19)
chr1:39896374-39896580 
HIGH PSI
HsaEX0037156
(MACF1)
Rat
(rn6)
chr5:141083317-141083523 
HIGH PSI
RnoEX0051758
(Macf1)
Cow
(bosTau6)
chr3:107226506-107226712 
HIGH PSI
BtaEX6018691
(MACF1)
Chicken
(galGal4)
chr23:5379938-5380144 
HIGH PSI
GgaEX6025257
([1])
Chicken
(galGal3)
chr23:5697285-5697491 
HIGH PSI
GgaEX6025257
(MACF1)
Zebrafish
(danRer10)
chr19:35491350-35491556 
HIGH PSI
DreEX0044254
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CCAGGAGAAGCTGGATGGGAT

				
R: 
CGAATTGGACCGAGAGCCATC

				
Band lengths: 
300-507

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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