BtaEX6008999 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23736658-23742632:-
Coord C1 exon
chr2:23742526-23742632
Coord A exon
chr2:23738341-23738462
Coord C2 exon
chr2:23736658-23736841
Length
122 bp
Sequences
Splice sites
3' ss Seq
TGTGTCCCGCCACTCTTCAGACA
3' ss Score
6.68
5' ss Seq
ATGGTAAGA
5' ss Score
9.48
Exon sequences
Seq C1 exon
GTTCCTTCAGAGAAGATCCTCAGGGCTGGAAAAATTTTACGAAACGCCATTCTCTCTCGAGCACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAG
Seq A exon
ACAGTGCTGTGTGGGGACCGAGCTGGTGGACTGGATGCTGCAGCAGACTCCATGTGTTCACTCCCGAACGCAAGCTGTGGGCATGTGGCAAGTCCTGGTAGAAGACAGCGTTCTCAACCATG
Seq C2 exon
TGGATCAGGAGCATCACTTCCAAGACAAATATTTATTTTATCGGTTTCTGGATGATGAGCATGAGGACGCCCCTTTGCCTACGGAGGAGGAAAAAAAGGAGTGTGATGAGGAGCTGCAGGACACCATGCTGCTGCTGTCGCAGATGGGCCCGGACGCCCACATGAGGATGGTCCTTCGCAAACC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'20-18,'20-17,23-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.274
Domain overlap (PFAM):
C1:
PF0061016=DEP=PU(19.7=38.9)
A:
PF0061016=DEP=FE(57.7=100)
C2:
PF0061016=DEP=PD(21.1=24.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGAAACGCCATTCTCTCTCG
R:
GACCATCCTCATGTGGGCGT
Band lengths:
242-364
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]