DreEX0060850 @ danRer10
Exon Skipping
Gene
ENSDARG00000079872 | rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:ZFIN;Acc:ZDB-GENE-070912-385]
Coordinates
chr9:2974056-2977570:-
Coord C1 exon
chr9:2977464-2977570
Coord A exon
chr9:2975734-2975855
Coord C2 exon
chr9:2974056-2974239
Length
122 bp
Sequences
Splice sites
3' ss Seq
TGTGTGTGTGTGTGTTGTAGGCG
3' ss Score
10.94
5' ss Seq
ACGGTAAAC
5' ss Score
5.88
Exon sequences
Seq C1 exon
GTGCCGTCAGAGAAGATACTTCGGGCAGGAAAAGTGCTACGAAACATCATTTTATCTCGAGCGCCTCATATGATAAGAGACAGGAAATACCACCTGAAGACGTACAG
Seq A exon
GCGGAGCTGCATCGGCACTGAGCTCGTTGATTGGCTGTTGCAGCACACATCATGTGTTCACTCTCGTGTTCAGGCTGTAGGGATGTGGCAGGTGCTGCTGGAGGAGGGAGTGCTTAACCACG
Seq C2 exon
TGGACCAGGAGCCGACTTTCCAAGATAAATATCTGTTTTATCGCTTTCTGGATGACGAAAGTGAGGATTCAGGAATGCCGAGTGAGGAAGACAAGAAGGAGAGTGACGAAGAGCTGCAGGACACGCTTCTGCTGCTGTCTCAGATCGGCCCTGATGCTCATCTGCGGATGATCCTGCGCAAACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079872-'10-10,'10-9,11-10
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.194
Domain overlap (PFAM):
C1:
PF0061016=DEP=PU(19.7=38.9)
A:
PF0061016=DEP=FE(57.7=100)
C2:
PF0061016=DEP=PD(21.1=24.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCGGGCAGGAAAAGTGCTAC
R:
ATCAGGGCCGATCTGAGACAG
Band lengths:
243-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]