MmuEX6102155 @ mm10
Exon Skipping
Gene
ENSMUSG00000049044 | Rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72174802-72180103:+
Coord C1 exon
chr2:72174802-72174908
Coord A exon
chr2:72178178-72178299
Coord C2 exon
chr2:72179920-72180103
Length
122 bp
Sequences
Splice sites
3' ss Seq
CCATGTCTTATCTCCTTTAGACA
3' ss Score
7.58
5' ss Seq
ATGGTAAGA
5' ss Score
9.48
Exon sequences
Seq C1 exon
GTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCACCTAAAGACATACAG
Seq A exon
ACAATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTGTTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATG
Seq C2 exon
TGGACCAGGAGCGCCATTTCCAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCTTTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGATGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAAACC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'42-31,'42-23,51-31=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.006 A=0.000 C2=0.210
Domain overlap (PFAM):
C1:
PF0061016=DEP=PU(19.7=38.9)
A:
PF0061016=DEP=FE(57.7=100)
C2:
PF0061016=DEP=PD(21.1=24.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCATTCTCTCTCGAGCTCC
R:
TCGCAGGATCATTCTCATGTGG
Band lengths:
242-364
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types