MmuEX6102155 @ mm9
Exon Skipping
Gene
ENSMUSG00000049044 | Rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72012859-72018160:+
Coord C1 exon
chr2:72012859-72012965
Coord A exon
chr2:72016235-72016356
Coord C2 exon
chr2:72017977-72018160
Length
122 bp
Sequences
Splice sites
3' ss Seq
CCATGTCTTATCTCCTTTAGACA
3' ss Score
7.58
5' ss Seq
ATGGTAAGA
5' ss Score
9.48
Exon sequences
Seq C1 exon
GTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCACCTAAAGACATACAG
Seq A exon
ACAATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTGTTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATG
Seq C2 exon
TGGACCAGGAGCGCCATTTCCAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCTTTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGATGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAAACC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'22-18,'22-16,23-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.007 A=0.000 C2=0.210
Domain overlap (PFAM):
C1:
PF0061016=DEP=PU(19.7=38.9)
A:
PF0061016=DEP=FE(57.7=100)
C2:
PF0061016=DEP=PD(21.1=24.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCATTCTCTCTCGAGCTCC
R:
TCGCAGGATCATTCTCATGTGG
Band lengths:
242-364
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: