BtaEX6018687 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107230114-107234861:-
Coord C1 exon
chr3:107234691-107234861
Coord A exon
chr3:107233887-107234053
Coord C2 exon
chr3:107230114-107230273
Length
167 bp
Sequences
Splice sites
3' ss Seq
TCTTTAAACATGTACATCAGTTG
3' ss Score
1.65
5' ss Seq
CAGGTAAAG
5' ss Score
9.65
Exon sequences
Seq C1 exon
GTTGCACAAAGAATCGCACAACTGCAAGAGGCTTTGTTGCATTGTGGGAAGTTCCAAGATGCCTTGGAGCCATTGCTCAGCTGGTTGGCAGACACTGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCTGAGTATAAAGTGGTGAAAGCCCAGATCCAAGAACAGAAG
Seq A exon
TTGCTCCAGAGGCTACTAGATGATCGAAAGGCCACAGTAGACATGCTTCAAGCAGAAGGAGGCAGAATAGCACAGTCAGCTGAGCTGGCTGATAGAGAGAAGATCACTGGACAGTTGGAGAGTCTTGAAAGTAGATGGACTGGACTACTCAGCAAGGCAACAGCCAG
Seq C2 exon
GCAGAAACAGCTGGAAGATATCCTGGTTCTGGCCAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTCGCCAACTCAGAACCTGTTGGCACCCAGACTGCTAAAATACAGCAGCAGATCATTCGGCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024-'73-73,'73-72,74-73=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.018 A=0.011 C2=0.109
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
A:
PF0043516=Spectrin=FE(51.4=100)
C2:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCTTTGTTGCATTGTGGGA
R:
GGTTCTGAGTTGGCGAGCTTT
Band lengths:
254-421
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]