RnoEX6023222 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141086981-141090821:-
Coord C1 exon
chr5:141090651-141090821
Coord A exon
chr5:141088766-141088932
Coord C2 exon
chr5:141086981-141087140
Length
167 bp
Sequences
Splice sites
3' ss Seq
TTTAAACATACGTGTCACAGTTG
3' ss Score
3.29
5' ss Seq
CAGGTAAGG
5' ss Score
11.08
Exon sequences
Seq C1 exon
GTTGCACAAAGAATTGCACAACTGCAGGAGGCTTTGTTACATTGTGGGAAATTTCAAGATGCCTTAGAGCCATTGCTCAGCTGGTTGACAGACACCGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCCGAGTATAAAGTGGTGAAAGCCCAGATCCAAGAACAGAAG
Seq A exon
TTGCTCCAGCGACTCCTAGATGATCGCAAGGCCACAGTGGATATGCTTCAAGCAGAAGGAGGCAGAATAGCCCAGGCTGCTGAACTGGCTGATAGAGAGAAAATCACTGGGCAGCTGGAAAGCCTTGAATGTAGATGGACTGAGCTCCTCAGCAAGGCAGCAGCCAG
Seq C2 exon
GCAAAAGCAGCTGGAGGATATCTTGGTTCTGGCTAAACAATTCCACGAGACAGCTGAGCCTATTTCTGACTTCCTATCTGTCACAGAGAAAAAGCTTGCTAACTCGGAACCTGTTGGCACTCAAACTGCCAAAATACACCAGCAGATTATTCGTCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'90-100,'90-99,92-100=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.018 A=0.000 C2=0.093
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
A:
PF0043516=Spectrin=FE(51.4=100)
C2:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCATTGCTCAGCTGGTTGACA
R:
TCTGCTGGTGTATTTTGGCAGT
Band lengths:
248-415
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]