DreEX6073723 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35495743-35497981:-
Coord C1 exon
chr19:35497811-35497981
Coord A exon
chr19:35496056-35496222
Coord C2 exon
chr19:35495743-35495902
Length
167 bp
Sequences
Splice sites
3' ss Seq
AAAATCCCATGTTTTATAAGCTT
3' ss Score
3.36
5' ss Seq
CAGGTAAAC
5' ss Score
7.82
Exon sequences
Seq C1 exon
GTTGCTGAAAGAATCGCACAGCTGCAAGAGGCCCTGTTGCATTGTGGGAAGTTTCAAGACGCCCTGGAGCCACTCTTGAGCTGGCTGAGTGACACAGAGGAACTGATAGCCAATCAGAAACCTCCATCTGCTGAGTACCGTGTTGTCAAGGCACAAATTCAGGAGCAAAAG
Seq A exon
CTTCTGCAACGGTTGCTGGATGACCGCCGTGGGACTGTAGAGATGATCAGGGCCGAAGGAGAGCGTATCGCTGCTACAGCCGAAACACAAGACCGAGACAAAATCCAGAAACAGCTTCAGAGTTTAGGAGAAAGATGGACAAACCTGCTGGAAAAGGCCAGCGCCAG
Seq C2 exon
GCAGAAGCAGCTCGAGGAACTGCAGGTGCTGGCATTACAGTTCCATGAGGCCGTGGAGCCGCTCAGTGAGTGGCTGAGCGCAACAGAGAGACGCCTGAGCTCTGCTGAGCCAATGGGAACCCAGACATCCAAAATCACACAGCAGATTGTAAGGCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'72-75,'72-74,73-75=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.035 A=0.321 C2=0.333
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
A:
PF0043516=Spectrin=FE(51.4=100)
C2:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTGGGAAGTTTCAAGACGCC
R:
GTTCCCATTGGCTCAGCAGAG
Band lengths:
249-416
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]