MmuEX6095874 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123074991-123078806:-
Coord C1 exon
chr4:123078636-123078806
Coord A exon
chr4:123076586-123076752
Coord C2 exon
chr4:123074991-123075150
Length
167 bp
Sequences
Splice sites
3' ss Seq
TTTATACACACATGCCACAGTTG
3' ss Score
3.21
5' ss Seq
CAGGTACGG
5' ss Score
10.88
Exon sequences
Seq C1 exon
GTTGCACAAAGAATTGCACAACTGCAGGAGGCTCTGTTACATTGTGGGAAATTTCAAGATGCCTTAGAGCCTTTGCTCAGCTGGTTGACAGATACTGAGGAGCTCATAGCCAATCAGAAGCCTCCATCTGCTGAGTATAAAGTGGTGAAAGCACAGATCCAAGAACAGAAG
Seq A exon
TTGCTCCAGCGACTCCTAGATGATCGAAAGGCCACAGTGGATATGCTTCAAGCAGAAGGAGGCAGAATAGCCCAGTCAGCTGAGCTGGCTGATAGAGAGAAAATCACTGGGCAGCTGGAGAGTCTTGAACGTAGATGGACTGATCTCCTCAGCAAGGCAGCAGCCAG
Seq C2 exon
GCAAAAACAGCTGGAAGATATCTTGGTTCTGGCTAAACAATTCCATGAGACAGCTGAGCCTATTTCTGACTTCTTATCTGTCACAGAGAAAAAGCTTGCTAACTCGGAACCTGTTGGCACTCAGACTGCCAAAATACACCAGCAGATTATTCGTCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'80-78,'80-77,81-78=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.018 A=0.022 C2=0.102
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(8.3=15.8),PF0043516=Spectrin=PU(41.1=77.2)
A:
PF0043516=Spectrin=FE(51.4=100)
C2:
PF0043516=Spectrin=PD(5.6=11.1),PF0043516=Spectrin=PU(41.1=81.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTTTGCTCAGCTGGTTGACA
R:
TGCTGGTGTATTTTGGCAGTCT
Band lengths:
246-413
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: