BtaEX6077438 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000003132 | CHRNB4
Description
Bos taurus cholinergic receptor, nicotinic, beta 4 (CHRNB4), mRNA. [Source:RefSeq mRNA;Acc:NM_174517]
Coordinates
chr21:31525484-31537227:-
Coord C1 exon
chr21:31537173-31537227
Coord A exon
chr21:31532064-31532212
Coord C2 exon
chr21:31525484-31525528
Length
149 bp
Sequences
Splice sites
3' ss Seq
CTGACTGTGTGCTTTCCCAGGGG
3' ss Score
8.76
5' ss Seq
GTGGTAGGT
5' ss Score
7.23
Exon sequences
Seq C1 exon
ATGAGAAGCGCTCTCCCTCTGGTCCTTTTCTCACTGGTTGCCCTTTGCGGGCGAG
Seq A exon
GGGACTGCCGTGTGGCCAATGCAGAGGAGAAGCTCATGGACGACCTTCTGAACAAAACCCGCTACAACAACTTGATCCGCCCAGCCACCAGCTCCTCCCAGCTCATCTCCATCCAGCTGCAGCTCTCCCTGGCCCAGCTCATCAGCGTG
Seq C2 exon
AATGAGCGAGAACAGATCATGACCACCAACATCTGGCTGAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000003132-'0-1,'0-0,1-1=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(19.9=82.0)
C2:
PF0293118=Neur_chan_LBD=FE(6.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGCTCTCCCTCTGGTCCTTTT
R:
CCAGATGTTGGTGGTCATGATCT
Band lengths:
83-232
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]