DreEX6066993 @ danRer10
Exon Skipping
Gene
ENSDARG00000101677 | chrnb4
Description
cholinergic receptor, nicotinic, beta 4 (neuronal) [Source:ZFIN;Acc:ZDB-GENE-120809-1]
Coordinates
chr18:50630376-50633195:+
Coord C1 exon
chr18:50630376-50630607
Coord A exon
chr18:50632753-50632898
Coord C2 exon
chr18:50633151-50633195
Length
146 bp
Sequences
Splice sites
3' ss Seq
GTGTGTGTGTGTGTGTGCAGGTG
3' ss Score
10.65
5' ss Seq
GTGGTCAGT
5' ss Score
4.85
Exon sequences
Seq C1 exon
CGCGCGCATGGAGGAGACGCGCACGGACACGCGCAGCCGCCGCCGCCGGTCCGAGCGCTCCCCATCCTCATCAATGCTGTTGGCTTTTCTGAACTCCTGTGTGTGTGTATGTGAATGAATGGCGGTGTGTGTGTGTGAGGCGGAATGGGACTGAAGAGATGCAGAATCCCGGCCGGATCCAGCATGACGCGCGCGCTCTGCGTCCTCCTGTGCGCCCTCAGCCTCGCCAGAA
Seq A exon
GTGGTGTGTGTGCAGACTCAGAGGAGCGTCTGATGAACTGGCTGCTGGGTAAACATCGCTACAATAAACTGATCCGGCCGGCGCTGAACCGCACCGAGAGAGTGACCGTCCTGCTGCAGGTGTCTCTGGCCCAGCTCATCAGTGTG
Seq C2 exon
AATGAAAGAGAGCAGATCATGACCACAAACGTCTGGCTCACTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000101677-'0-2,'0-1,2-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(19.9=83.7)
C2:
PF0293118=Neur_chan_LBD=FE(7.3=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATGGAGGAGACGCGCAC
R:
ACGTTTGTGGTCATGATCTGCT
Band lengths:
258-404
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]