MmuEX6066068 @ mm10
Exon Skipping
Gene
ENSMUSG00000035200 | Chrnb4
Description
cholinergic receptor, nicotinic, beta polypeptide 4 [Source:MGI Symbol;Acc:MGI:87892]
Coordinates
chr9:55036587-55048779:-
Coord C1 exon
chr9:55048444-55048779
Coord A exon
chr9:55043777-55043925
Coord C2 exon
chr9:55036587-55036631
Length
149 bp
Sequences
Splice sites
3' ss Seq
CTGACTATACGATTTCCCAGGGG
3' ss Score
5.03
5' ss Seq
GTGGTAGGT
5' ss Score
7.23
Exon sequences
Seq C1 exon
AGCTAGTCCACCCTCCCGAGTGCCACGCACTACCCGGCTCCTGAGTGTGAGAAAGTCTCGGGACCATCATCCATAGCCACAGGTTCCCCCAGACACACCCTGCTCTGGGTCTACCCCAAACTCCAGTTCAGTCCTCTAAGTGGGTGGGACCCACAGCAGCTTCCCAGGTGGGGAGCACAGGCTCCATCGGGTCCTAGTGCCGCCGGGCACAGAGAGACTGCCAGCTGGGACAGACGCTCACTCGCGGTTCCATTGTGGGGTGACCGGCAGCCACCCGGCCGGCCATGAGGGGTACGCCCCTGCTCCTCGTCTCTCTGTTCGCTCTGCTTCAGCCTG
Seq A exon
GGGACTGCCGCCTGGCCAACGCAGAGGAGAAGCTGATGGATGATCTCCTGAACAAAACCCGCTACAACAACCTGATCCGCCCAGCCACCAGCTCCTCCCAGCTCATCTCCATCCGCCTGGAGCTATCACTGTCCCAGCTCATCAGTGTG
Seq C2 exon
AATGAGCGAGAACAGATCATGACCACCAGCATCTGGCTGAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035200-'1-6,'1-2,4-6=AN
Average complexity
A_C1
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(19.9=82.0)
C2:
PF0293118=Neur_chan_LBD=FE(6.8=100)
Main Skipping Isoform:
ENSMUST00000034854fB7750
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTCCTCTAAGTGGGTGGGAC
R:
CCAGATGCTGGTGGTCATGAT
Band lengths:
243-392
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types