BtaINT0067981 @ bosTau6
Intron Retention
Gene
ENSBTAG00000047135 | GORASP1
Description
Bos taurus golgi reassembly stacking protein 1, 65kDa (GORASP1), mRNA. [Source:RefSeq mRNA;Acc:NM_001046518]
Coordinates
chr22:12474052-12475848:-
Coord C1 exon
chr22:12475645-12475848
Coord A exon
chr22:12474139-12475644
Coord C2 exon
chr22:12474052-12474138
Length
1506 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
3' ss Seq
TCGTGATTCTTTTTCCGCAGGAT
3' ss Score
9.48
Exon sequences
Seq C1 exon
AACAAGGAGAATGACACGCTCAAGGCCCTGCTGAAGGCCAACGTGGAGAAGCCAGTGAAGCTGGAGGTGTTCAACATGAAGACCATGAAAGTGCGTGAGGTGGAGGTGGTTCCCAGCAACATGTGGGGCGGCCAGGGCCTGCTGGGCGCCAGCGTCCGCTTCTGCAGCTTCCGCCGGGCCAGCGAGCACGTGTGGCATGTGCTG
Seq A exon
GTGAGTGGGGCGTGGCGGTGGGATCACTGGGGCAGACGGGGATGGGTGTGTGGGGAGGCCTCCTAGGAAAGGCCGGCAGCCACTGGGCCTGTCTGGGGATTCTGAGGGCAGGGTGTGGTCTCCTTGTTCCAGGGTCCCAGAGAGGGCAACTCCTCGTCACCTGAACAGGGCCCTCTGAATTTGGGTAATTTCTTACACTCCAGACCTTGGTTTCTTCTTTGGAAAGATGGGGCCGCCATCTGCTGGAAGGGTCGTTGTGCTGCCAGAATGCTGCTGTTACTGCTTTGCATTGTTTAGTTGCTAAGTCGTGTCTGATTCTGCAACCCCGTGGACTGTAGCCCACCAGACTCCTCTGTCCATGGGAGTCTCCAGGCAAGAACACTGGAGTGAGCTGCCATGCCCTTCTCCAGGGGATCTTCCCGACCCAGGGATTGAACCTGTATCTCCTGCATTGGCAGGCAGATTCTTTACCGCTGTGCCACCTGGGAAATCCTGCTGCTGTGCTTTAGTCCTTATCTAAAAAGAGTGTTTTTTTCTGGTATACTCTAGGGTATTACCAGTATCATGTTTTTTTGGGCAGGTCCCCTGAGAGCCCCTTCGGGCACTCGACTGGTGCTTGGGGTCTGAACCTTAATGAGTCTCCGTGACTGAGCTGTCACAGCTGTTGCTGTTAGTGTTCATTCCTAGACATACACGCACACATGTGTGACATGCACAGTCACTGATTGTGCCCCGGTTCTTGAATGCACAGTCCTGCAGCTTGATGGGCAGGAGGAGCCCTGAGCTGGAAATGGGCTGATTTGGTTTCTAGTTGCTTCTGTTGCTCACTGACTCCTTGGGGGAGTCTGCTCAACCTTTTCAGCTGGCCTTACTCTGTCCATGAGTGCAGTGCAGGGGTGATGATGCAAATCCTTCCACGTTTCTTGGGAGACTGGGATCGGGGAATGGAGCTTCCCAAGGGAGAAGGCAATCCTGGCACCTACTTATCACCACCCTCGCCTTTAACCTTTTAACCTCCCCCCTGTCCCAAGGTCTTCAGCCCTATGCCTGCAGCAGTAACAGCAATAAAGTGAGGTATTTACTGTGGGACAAGGTGTGGTTTTCATTCTGTACATGTATTATTATTTTTTTTTGGTCTTCTCAACAACCTTTTGCCTAAGTACTATATATCCTACTTTTAACTGTGGGGAAACTGGGGCAGAGGAGTAAAGTGTCTGTCTTCAGTGCCCCAGCTCGCAGCGGGGGGAGGCTGGACTTGGATCGGGTGGTCAGATGCGGGAGCTGTAAGGTGAGCCGTGACACAGTGCTGCCCTCTGCTGGGCAGACTGTGGACTGGGGGAGGCAGGGGAACAGGGGCTGGGTGGGGCTGGGCGAGTTTGGGTTTGAGGTCTTGGCCTGGGGATGGAGGACAGGTATACTACTCACCCCCTGAAGACACAGCTCTCCACCTGCTGCCCCCCAGTAGCAAGGCTGACAGTTGGATAATTCGTGATTCTTTTTCCGCAG
Seq C2 exon
GATGTGGAACCCTCTTCACCTGCTGCCCTCGCAGGCCTGCGCCCCTACACAGACTACGTTGTGGGCTCGGACCAGATCCTCCAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000047135:ENSBTAT00000065507:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF044959=GRASP55_65=PU(34.3=69.1)
A:
NA
C2:
PF044959=GRASP55_65=FE(20.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGAGAATGACACGCTCAAGG
R:
GATCTGGTCCGAGCCCACAA
Band lengths:
278-1784
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]