BtaINT0077553 @ bosTau6
Intron Retention
Gene
ENSBTAG00000001518 | ISG20L2
Description
Bos taurus interferon stimulated exonuclease gene 20kDa-like 2 (ISG20L2), mRNA. [Source:RefSeq mRNA;Acc:NM_001046217]
Coordinates
chr3:14154780-14157648:+
Coord C1 exon
chr3:14154780-14155628
Coord A exon
chr3:14155629-14157447
Coord C2 exon
chr3:14157448-14157648
Length
1819 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
3' ss Seq
CTCTTCCATGTCTGTTTCAGATC
3' ss Score
11.78
Exon sequences
Seq C1 exon
GGTATGCTGCAAACCTCCAAATGCTGGTCTGTGGGATTCAGCAGATGAGTCTTCTATCTTCCTCTCATCTGTAAGCTGTTCTTCCCAACTGTTCTCTCACCATCCAGTGTCATCATGTCTACCTTACTCCTCAATCTGGATTTTGGCGAGCCTCCCCCCAAAAAGGCATTAGAGGGAAATGCCAAGCACCGAAAATTTGTCAAGAAGAGGCGACTGTTGGAACGGAAAGGCTTCCTCAATAAGAAGAAGCAGCCCCCTAGCAAGGTGCCTAAGTTGCACTCAGAACCTTCACAGAAAGGGGAAACTCCCAGGGTAGATGGCACTTGGAAGGCCACTCCCCTTCCAAAGAAGAAGACAACAGCTGCCTCCAGCAGTGGGTCAGAGCAGTCCCTGGACAAGAAAGCTGCAGTGCCTTGGCTGACTCCTGCCCCTTCACAGAAGGCTGGTTCTGTTGTGGCTAAAGTAGATTTGCTGGGGGAGTTCCAGAGCGCCCTACCAAAGATTAAGAGCCACCCAACTCGCCCCCAGAAGAAGGGATCCCAGAAGAATCCTCCACCCAAGAATGGCCCACAGAACTCCACCCATACACATTCAGAGAATAAATACTCTGGGGTATCCCAGAAGATACCGGGGAAGATGGTGGCAATTGACTGTGAGATGGTGGGTACTGGACCCAAGGGGCATGTCAGTTCCTTGGCTCGATGTAGCATTGTCAACTACGACGGAGATGTGCTCTATGATGAGTACATCCTCCCCCCCTGCCACATTGTGGACTACCGGACCAGATGGAGTGGTATCCGGAAGCAGCACATGGTGAATGCTACACCCTTCAAGATTGCTCGGAACCAG
Seq A exon
GTAGGAGGCATGAGGGATGGTGGTAGCAGCGAAGCAGATAGGGCAAGGTCCAAATCATTTTTCAGCAAAGGATATCACTCTGGGAAGGCACCAAAGGATTGTGGAGGGATGGTTGGAGTGGATGGGAGTAGCAGATAAAGAAGGCTTTTCTTTTGGGATGAAGAGTGGTAAGAACCTGGAAACTTACAAATAGCTGGTGTAATAAACTGTATTAGATCTGTTTTAGATCCCAAGTGGAATCCTCATCTGGCTAATTGGGACTCTGCTAGAAAAACAGCAACCTGTGATGGACAGAGTAGCAGGCATGAGTGCCAGGGGGAAAGGGCTCAATTTGAGAATACCTTGTAGAGGTGGGGCTGGGGTTGAGAACAGAGAGGTGGAGGGCGCTTCCCAAGCAAGAAGTGGCCTGTGGCAGTGTGGAAAGTTGGTGGATAACTGGAACAGGCAGCCTGCAGTGAAGGGGGTAAAAGACACAGGCCTGATATGCTGGGGTTCTCATCTCATTCACTGAACAATCCTAGCCACTGTAAGCTCTATGCTGAAAACTGGAGGCACGCTGAATGAACAGGAAAGGAGGATGAGCTGGTAGAGGGGAAGCGGAAAGGAGCTGCTGGTCTTGGAAGGCCTCCAGAGGGCACCCTTGATCCACTGTGTAATTTCAGCTCCACATTCTTTGTCTTGTCTAGACACACTGTTCCAGTCATTTCTCATATGCCAGAGCAGTAAGTTGGAGGAATTTCATCAGCTAGATTTTAGAAAGAACATGAAGAGTTAAAGAACATCCTGTGCACCCTCAAGTGTGAGCAAAGGCTCGGGAGAGTCAGAAGATCGGGTGGGTCTGAAACAGCACATCCACTTAGCTTTAATGAAGGCTGTGTGTTAAAATTAATGCAAAGTAAAGGTGTTAGTGGTCAGTAAACTGTAGGGGCGACTAAATACCAAGTTGATTAGACTATATCTGGTTTTCATCATAAAAATCAGTGCCCAAGCTGGGGTATGAGACCAAAGTACATTCAAATGAGTCTGTACCCTTCCAAGGGGGTCCCATTGAGAGGCCAGGCATTGATTCCAGGCCTGTTAACTCTTTCGCGGTCTGTTTGGAACCCCATTTTGGTAACAGCCCTCAGAGTTCATGGCATATTCTTTCGACATCCTCATTGGAAATGAATCTTTTTTTTTAGGGGGTGGGGCATACCTTGAGGCTTGTGAGATCTTTGTTCTGTAACTGGGGATCAAACCTGGTCCCCCTGGCAGTGAGAGTGCTGAGTCCTAACCACTGGACTGCCAATTCTTAATTCCCAATTTTTAATTTTTTATAGCTTGTAGGCCCTTTGAGAGTCTGATGAAAGCCATAGATTCCCTCCCAAAACAAACACAGAAACATACGCATATCAATTTGCATATAATTGGGGGCTTCCATTACCTCCCTGGAGCCCATCTTTACAGTAATTGTCCCAGCCTGTATCTAAGGCCAACGTAACAATTATCATGGTTAAAGAACTTCAAGTATCAAGCCAGGACTGAACTAGGTAATTACATGAACATCACATTGAGGTAGAAATTAATGCGACAATAAGGAACCACTGGCTAGGCGTCCAAACAGGGTTACACCATGAAAGATGCCTACTTTTACCTCAGGGAGAGGAGATGGATTTCAGGTAAAAGAAAACTTGTGGGAGAAGGCATTTCAGGGATGAGACAGGTGAGCAAGTGGTGAAAAGAGCCTAGGAGGACTTAGAGCTGTGTTGGACTTTTAGAATGCAGAGGCTGCATCTGTGAGTCGTGCTTCTTACCACCTCCTCTTCCATGTCTGTTTCAG
Seq C2 exon
ATCTTGAAGATTCTGGCCGGGAAGATAGTGGTGGGGCATGCCATCCACAACGACTTCAAAGCCCTTCAGTACATTCATCCCAAGTCCCTCACACGAGACACCTCTCATATCCCCCTCCTCAACCGTAAGGCTGACTGCCCAGAGAACGCCACCATGTCTCTGAAGAGTCTCACCAAGAAGCTGTTGAACCGGGACATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000001518:ENSBTAT00000001989:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.624 A=NA C2=0.015
Domain overlap (PFAM):
C1:
PF0092919=RNase_T=PU(44.2=28.2)
A:
NA
C2:
PF0092919=RNase_T=FE(42.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGATACCGGGGAAGATGGTGG
R:
CTTACGGTTGAGGAGGGGGAT
Band lengths:
356-2175
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]