DreINT0082684 @ danRer10
Intron Retention
Gene
ENSDARG00000102266 | isg20l2
Description
interferon stimulated exonuclease gene 20kDa-like 2 [Source:ZFIN;Acc:ZDB-GENE-070928-8]
Coordinates
chr16:29436871-29439743:-
Coord C1 exon
chr16:29439072-29439743
Coord A exon
chr16:29437072-29439071
Coord C2 exon
chr16:29436871-29437071
Length
2000 bp
Sequences
Splice sites
5' ss Seq
GAGGTATGA
5' ss Score
8.55
3' ss Seq
ATTATATGTTTTGCACACAGATT
3' ss Score
7.55
Exon sequences
Seq C1 exon
AGCAAAAAGACATCAAGAAGACACTTTTTGTAGTCGACTTTTGATGGTATAAGAATGTCAGGGATGATGTTCAACAGGTATTGTGAGCCTAGACAAGGAGGTCGATCAGGAAAATCCAGAGGAAAATCCAAACAAGATCGTTTTCTTTCACGAAGGCGTTACCTGGAGAGCGAAGGTTTGATAACTGAGAAACAAAATCAAAAGCAACCTGAAAAGATCCAACCGAAGAAGACGAAAAAAAAGCCCAGAAATGTCAGGTTCAACGTAGGAGAGCATTCAAAACCACGTGCGTTTTTTCCTAAATTTACCTCTTATCTGCCTAATAGCACCTATGAGCCTTCTAGTTCTGCACAAGCGTATTCCCGGACGTCCACATATTTATTTCCATCCCATGCCAACATATCTCAGAGGGATCTGACACAAAGAATGGCAGTTCCTCGCCCCCCTGGACCTATAAAATACTTGGCTCTGGACTGTGAGATGGTGGGAACAGGGCCGAAGGGGGCTCAGAGTGAACTGGCCCGCTGCAGTATTGTCTCCTATGATGGTGATGTGGTTTATGATAAATATGTCAAACCCATCAATCCAGTGACTGACTATCGCACTCGCTGGAGTGGAATCAGGAGGCAGGATCTACTCCACGCCACCCCTTTTTATCATGCGCAAAAAGAG
Seq A exon
GTATGAAATAAGCATCAGTAGCTAAATGATAAGGTTTCATTATGCTTAATTTTATGTCCACCTTTTCCTAACATTCCTAGTGTATTTTAAATTATGGCTTCCTCTTTTACTTTTGGTAACTTCACTTCAAAACATGAATATTTGCACTGCAATTAATCCATATGCTTAATGAAATCTCAAAGGTACAGTACGTTAACTTTATTTTTATCTGTCATGAATATAATGTTACACTTACAGTAGGTGACATTAATATTTACAGTAGTGGATGAGTAGACTCATCTCTGTTAAAAGCAATTAGTTTATTCTAACAAGGTTTTATTTTTGTATTTAGTGGCTGAATTGTATCTATTTTTAAAATAAAAATGTATCACTGATTGTTTACAGTTTAATGGACATTACTCCCATAATTCAGGCAAAGTGACTTGGAGTGTTGGACAATGGTGGATCGGACACTTTTATACAGGGTTTCAAAAGAGTCAAAATGTCTTAAATCTCAAAATCTAAATTTTACGCCTTAAAAAGTCTTAAAATTTACTGAAATATTCTGTTGTGGATCTTAAATCTTTCTAAAACAGGTCTTAATTTTCCTTTGTTGATAGTACAGTACAGTGAACTCATTGTCATGTTCAAGAAATCAGTCTGAGATGATTCGCATTTTATAAAATGACACGTTATCCTGCTGGAAGTAGCCATCAGAAGATGGGTACACTGTGGTCATAAAGAGATGGACATGGTCAGCAACAATACTCAGGTTGGCTGTGGCGTTGATATGATACTCAATTGGTTCTATTAATGTACGGAACCCCGGAAGGGACGTAGTGGAGGAGAAAAAAATTGGCTGGGTGAAAGAAAAAAATAGGTGGGAGGAAAATATATATTTCTACGTTTTTGCGTTCTCTCGCAAAGTTTTGCGTTCCCTCGCAAAGATGTTTTGCGTTCTCTCGCAAAACTGTTTCTCCACAAACACTACCTGTTCACTGCACTTACGTAAACCCTCCCGTTTTTGCCGAAAATTCTCCCGTATTTTACCCCACTTTCTTTACATTACTGTGCGCTGATCGTCGCCTTTCACTTTGCAACCTCTGAACCAGTGAATGCATGTATAAGCGCTGACTGACATTTTTTTCTATCACCCATTTTTTTTTTTTTTCACCCAGCCAACTTTTTTCTCCTCCACTACGTCCCTTCCGGGGTTCCGTACTAATGGGCCCAAAGTGTGCCAAGAAAATTTCCCCCACGCCATTACACCACCAGCAGCCTGAACCGATGATACTAGGCGGGATGCATTCACGCTTTGATGTTGTTGATGCCAAATTCTGACCCTACCATCCAAATTTCACAGCAAAAAATCGAGACTCATCAGACCAGGCAACGTTTTTCCAATCTTCTATCGTCCAACTTTGGTGAGCCTGTGTGAATTGTAGCCTCAGTTTCCTGTTCTTAGCTGACAGGAGTGGCACCCGGTGTGGACTTCTGCTGCTGTAGCCCATCTGCCTCAAGGTTTAACGCATTGTGTGTTCAAAGATGCTCCTCTACATACCTGGGTTGTAGTGAGTGTTTATTTGAGTTACTGTTTCCTTTCTTTTCACTGGAACGAGTCTGGCCATTCTCCTCTGGCATCAAGGCATTTGTGCCCACAAAATTGCTGCTCACTGGATATATTCTTTTTTTCAGACCATTCTCTGTAAACCCAAGAGATGATTGTGCGTGAAAATTCCAGTGCCTCAGCAGTTTCTGAAATATTCAGACCAGCCCATCTGGTCCACCACAACCATGCCACATTCAAAGTCACTTAAATCACCTTTCTGAACTGCAGCAGATCGTCTTTACCATGTCTAGATGCCTAAATGCATTGAGTTGCTGCCATGTGATTGGCTGATTGTGTTTATTCACTGGTTCCAAGTAAGATTATTCCAATCATTATTGCAGGTAATGGCCGACTAGTAATTGCTGACTGATTGAATAAATCTTCAAAACACTATTATATGTTTTGCACACAG
Seq C2 exon
ATTGTGAAGATAATAACAGGGAAGGTGGTTGTGGGACATGCTATCCATAATGACTTCAAGGCCCTAAAATATTTCCACCCCGCTTTCCAAACACGAGACACTTCGCGGATTCCACTGCTAAACGAGAAAGCTGGATTTCCTGAAAAACAATGTGTGTCTTTGAAGAAGCTCACCCAAGCTATCTTAAAGAGAGACATTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000102266:ENSDART00000167132:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.422 A=NA C2=0.015
Domain overlap (PFAM):
C1:
PF0092919=RNase_T=PU(44.2=33.5)
A:
NA
C2:
PF0092919=RNase_T=FE(42.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGAATGGCAGTTCCTCGCC
R:
GCGAAGTGTCTCGTGTTTGGA
Band lengths:
357-2357
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]