BtaINT0120966 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018928 | QARS
Description
glutamine--tRNA ligase [Source:RefSeq peptide;Acc:NP_001029640]
Coordinates
chr22:51487764-51488106:+
Coord C1 exon
chr22:51487764-51487849
Coord A exon
chr22:51487850-51488019
Coord C2 exon
chr22:51488020-51488106
Length
170 bp
Sequences
Splice sites
5' ss Seq
CAGGTGTGT
5' ss Score
6.99
3' ss Seq
TCCCATTCCCCTAATCTCAGGTT
3' ss Score
7.2
Exon sequences
Seq C1 exon
GTGAGAACTACAAGACCCCGGGCTATGTGACCACTCCTCATACCATGGATCTACTGAAGCAGCACCTGGACATCACCGGAGGACAG
Seq A exon
GTGTGTGGCAGTATTGGCAGGCAAGCTATGCCATCATCACTTGCCTGCGGTTTGGGCTGCCTACATGCTACTCCACCTTACCTGTCATGATGGCCTCCCCAGGAAGGGTCATCCTTTAGTATAGTCTGAGCTCTGACCTGCTTCCTCTGCTCCCATTCCCCTAATCTCAG
Seq C2 exon
GTTCGTACCCGGTTCCCGCCAGAACCCAATGGAATCCTGCACATTGGACATGCCAAAGCAATCAATTTCAATTTTGGCTATGCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018928:ENSBTAT00000025191:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.138 A=NA C2=0.103
Domain overlap (PFAM):
C1:
PF0455710=tRNA_synt_1c_R2=PD(22.6=72.4),PF0074916=tRNA-synt_1c=PU(0.1=0.0)
A:
NA
C2:
PF0074916=tRNA-synt_1c=FE(9.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAGAACTACAAGACCCCGGG
R:
CCAAAATTGAAATTGATTGCTTTGGC
Band lengths:
162-332
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]