Special

MmuINT0130128 @ mm9

Intron Retention

Gene
Description
glutaminyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:1915851]
Coordinates
chr9:108413662-108413949:+
Coord C1 exon
chr9:108413662-108413747
Coord A exon
chr9:108413748-108413862
Coord C2 exon
chr9:108413863-108413949
Length
115 bp
Sequences
Splice sites
5' ss Seq
CAGGTGTGT
5' ss Score
6.99
3' ss Seq
TATCCCATTCCTTTACTTAGGTA
3' ss Score
7.69
Exon sequences
Seq C1 exon
GTGAGAACTACAAGACGCCAGGCTATGTGATCACTCCATATACCATGGATCTGCTGAAGCAGCACCTGGAGATCACTGGGGGACAG
Seq A exon
GTGTGTGGGGGTGTGGCCAGGCAGACACTTGACTGCCTAGGCTGCCTCATCTCATCTATTTCAGTAGGTGATCTGAGCTTTGGTCCCATTCCCTTTATCCCATTCCTTTACTTAG
Seq C2 exon
GTACGTACGCGGTTCCCTCCAGAGCCCAATGGAATCCTGCATATTGGACACGCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032604-Qars:NM_133794:9
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=NA C2=0.034
Domain overlap (PFAM):

C1:
PF0455710=tRNA_synt_1c_R2=PD(22.6=72.4),PF0074916=tRNA-synt_1c=PU(0.1=0.0)
A:
NA
C2:
PF0074916=tRNA-synt_1c=FE(9.3=100)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAGAACTACAAGACGCCAGG
R:
GGCATAACCAAAGTTGAAATTGATGG
Band lengths:
170-285
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types