GgaINT1023089 @ galGal4
Intron Retention
Gene
ENSGALG00000006853 | QARS
Description
glutaminyl-tRNA synthetase [Source:RefSeq peptide;Acc:NP_001012800]
Coordinates
chr12:11583261-11583560:+
Coord C1 exon
chr12:11583261-11583346
Coord A exon
chr12:11583347-11583473
Coord C2 exon
chr12:11583474-11583560
Length
127 bp
Sequences
Splice sites
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
3' ss Seq
TTCTCTTTTCCCCCACACAGGTG
3' ss Score
11.44
Exon sequences
Seq C1 exon
GAGAGAACTACAAAACAGAGGGCTACGTGGTGACACCCAACACCATGGCTCTGCTGAAGCAGCACCTGGCGCTCACGGGTGGGCAG
Seq A exon
GTGAGGTCAGGTCTCCGAGCACGGCGACTCCTTTGAGGGTGACATCATCGTGTAAGGCTGCAGCGGGCTCTGGGGAGGCACAGAGTGTGGGGGATTGTGTCAGTGCCTTCTCTTTTCCCCCACACAG
Seq C2 exon
GTGCGGACGCGGTTCCCTCCTGAGCCCAATGGCATTCTGCACATCGGCCATGCCAAGGCAATCAACTTCAACTTTGGTTACGCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000006853:ENSGALT00000011085:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.034 A=NA C2=0.138
Domain overlap (PFAM):
C1:
PF0455710=tRNA_synt_1c_R2=PD(22.8=72.4),PF0074916=tRNA-synt_1c=PU(0.1=0.0)
A:
NA
C2:
PF0074916=tRNA-synt_1c=FE(9.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGAGAACTACAAAACAGAGGGCT
R:
GGCGTAACCAAAGTTGAAGTTGA
Band lengths:
170-297
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]