MmuINT0130128 @ mm10
Intron Retention
Gene
ENSMUSG00000032604 | Qars
Description
glutaminyl-tRNA synthetase [Source:MGI Symbol;Acc:MGI:1915851]
Coordinates
chr9:108511331-108511618:+
Coord C1 exon
chr9:108511331-108511416
Coord A exon
chr9:108511417-108511531
Coord C2 exon
chr9:108511532-108511618
Length
115 bp
Sequences
Splice sites
5' ss Seq
CAGGTGTGT
5' ss Score
6.99
3' ss Seq
TATCCCATTCCTTTACTTAGGTA
3' ss Score
7.69
Exon sequences
Seq C1 exon
GTGAGAACTACAAGACGCCAGGCTATGTGATCACTCCATATACCATGGATCTGCTGAAGCAGCACCTGGAGATCACTGGGGGACAG
Seq A exon
GTGTGTGGGGGTGTGGCCAGGCAGACACTTGACTGCCTAGGCTGCCTCATCTCATCTATTTCAGTAGGTGATCTGAGCTTTGGTCCCATTCCCTTTATCCCATTCCTTTACTTAG
Seq C2 exon
GTACGTACGCGGTTCCCTCCAGAGCCCAATGGAATCCTGCATATTGGACACGCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032604:ENSMUST00000006838:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.017 A=NA C2=0.051
Domain overlap (PFAM):
C1:
PF0455710=tRNA_synt_1c_R2=PD(22.6=72.4),PF0074916=tRNA-synt_1c=PU(4.8=13.8)
A:
NA
C2:
PF0455710=tRNA_synt_1c_R2=PD(10.0=26.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAGAACTACAAGACGCCAGG
R:
GGCATAACCAAAGTTGAAATTGATGG
Band lengths:
170-285
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types