BtaINT0157566 @ bosTau6
Intron Retention
Gene
ENSBTAG00000014270 | UNC5B
Description
Bos taurus unc-5 homolog B (C. elegans) (UNC5B), mRNA. [Source:RefSeq mRNA;Acc:NM_001099029]
Coordinates
chr28:27589165-27589577:+
Coord C1 exon
chr28:27589165-27589329
Coord A exon
chr28:27589330-27589544
Coord C2 exon
chr28:27589545-27589577
Length
215 bp
Sequences
Splice sites
5' ss Seq
AAAGTGAGT
5' ss Score
8.4
3' ss Seq
CCCTTTATTTCCCTCGACAGATA
3' ss Score
11.19
Exon sequences
Seq C1 exon
TCGACGGCGGGTGGACGGAGTGGAGCAAGTGGTCAGCCTGTAGCACCGAGTGTGCGCACTGGCGCAGCCGCGAGTGCATGGCACCCCCACCCCAGAACGGAGGCCGGGACTGCAGCGGGACCCTGCTCGACTCCAAGAACTGCACTGATGGGCTGTGCGTACAAA
Seq A exon
GTGAGTCTTCAGAGGGCGGGGCCCAGGGTGGTGGCTCTGGAGCCAGAGAAGCCCCCCGCCCAGCCCCCACCGCCTGGCCTGCAGTGAAGCAGTCCCTTGGGGCCCTGCCCGCCCGCCAGGCTCTCATCTCATCCACGCATCTCCCCAGCACCCTCTCTACCACCCTCTCCGTCATCTGTGTCATTGTCTTTCTTTCCCTTTATTTCCCTCGACAG
Seq C2 exon
ATAAGAAAACTCTAAGTGACCCCAAAAGCCACC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014270:ENSBTAT00000018961:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.167
Domain overlap (PFAM):
C1:
PF150281=PTCRA=FE(47.4=100),PF0009014=TSP_1=WD(100=85.7)
A:
NA
C2:
PF150281=PTCRA=FE(9.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGTGGAGCAAGTGGTCAGC
R:
GGTGGCTTTTGGGGTCACTTA
Band lengths:
181-396
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]