DmeEX6006604 @ dm6
Exon Skipping
Gene
FBgn0032358 | Ppt2
Description
The gene Palmitoyl-protein Thioesterase 2 is referred to in FlyBase by the symbol DmelPpt2 (CG4851, FBgn0032358). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 2L:11280298..11281920. Its molecular function is described by: thiolester hydrolase activity; palmitoyl hydrolase activity; palmitoyl-(protein) hydrolase activity. It is involved in the biological process described with: macromolecule depalmitoylation. 8 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: viable; fertile. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, during early pupal stages, in adult male stages.
Coordinates
chr2L:11280298-11280928:+
Coord C1 exon
chr2L:11280298-11280515
Coord A exon
chr2L:11280617-11280758
Coord C2 exon
chr2L:11280821-11280928
Length
142 bp
Sequences
Splice sites
3' ss Seq
CTCATTTCGTCCGTTTGTAGTTT
3' ss Score
7.56
5' ss Seq
TGGGTAAGG
5' ss Score
9.08
Exon sequences
Seq C1 exon
GCAAGAAGAAGAGCGGCTAATAAAGATACGGATACATCAACAACAACAGTCGGAAATCCATTGTTCCCCACAAAATGAGACTTCGTTTGCAAGTCCTGGTGGCCCTGTTAACCTGCTCGTCCATTTCCGTCTCACTGGCCTACAAACCGGTGGTCATACTCCATGGAATTCTATCAGGAGCCGAATCGATGGCCTCTCTGGTCCGGGAAATCGAAGAG
Seq A exon
TTTCATCCTGGCACTATCGTCTACAACTGCGACAAGTTCAATGGATGGTACAGCTTGGAGAACGCCTGGCGACAGGTCGATCAGGTTAGGGATTATCTCAACGAGGTGGGCAAACTGCATCCAGAGGGCATCATTGTGCTGG
Seq C2 exon
GATACTCACAAGGAGGCCTTTTGGCCCGGGCGGCCATCCAAAGTCTGCCCGAGCACAATGTGAAGACCTTTATATCTCTATCCTCGCCACAGGCGGGGCAATACGGCA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0032358-'0-2,'0-1,1-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0208910=Palm_thioest=PU(10.1=56.2)
A:
PF0208910=Palm_thioest=FE(17.5=100)
C2:
PF0208910=Palm_thioest=FE(13.4=100)
Main Inclusion Isoform:
FBpp0079801
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGTCGGAAATCCATTGTTCCC
R:
AGGTCTTCACATTGTGCTCGG
Band lengths:
242-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)