DreEX0012157 @ danRer10
Exon Skipping
Gene
ENSDARG00000079388 | agrn
Description
agrin [Source:ZFIN;Acc:ZDB-GENE-030131-1033]
Coordinates
chr23:23831151-23925553:+
Coord C1 exon
chr23:23831151-23831357
Coord A exon
chr23:23921616-23921741
Coord C2 exon
chr23:23925426-23925553
Length
126 bp
Sequences
Splice sites
3' ss Seq
GTCTTCATCTCTCTCTTCAGGTA
3' ss Score
12.82
5' ss Seq
CATGTAAGT
5' ss Score
8.31
Exon sequences
Seq C1 exon
GGGGCTGCCCGAATGGTTGTAAGTTCAACGCCATATGTTTGCTGGATAATGGAGAGTTTCGGTGCTCCTGCGATCCCATACAATGCGACGGCACGTACAAGCCCCTGTGCGGAAAGGATGGCAAGACATACCCCAACGACTGCGAGAGGAAACTGCAGGAATGCCGGACCCAAAAAGACATCCCTGTCAAACAACAAGGGCCCTGTG
Seq A exon
GTACAACAGTAGCTACTACCACTCTTCCCACAGCTGAGGAGCGATCGTCCTGTGACAACACAGCGTTCGGCTGCTGTCCCGATGGGAAGACGGCCGCCGTCAACTCAGAGGGCACAAACTGCCCAT
Seq C2 exon
CCACCATGCGTTTCAGCGGCTTCCTGCACCTGGATAAGGTTGAGGGTCAGGAGGTCTTCTACACGCCTGAGATGGAGGACCCCAAATCGGAACTGTTTGGTGAAACGGCCCGAAGCATCGAGAGCGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079388_MULTIEX3-12/13=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.721 C2=0.435
Domain overlap (PFAM):
C1:
PF0005016=Kazal_1=WD(100=92.9)
A:
PF0139015=SEA=PU(0.9=2.3)
C2:
PF0139015=SEA=PU(38.2=97.7)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGAGAGTTTCGGTGCTCCT
R:
TTTGGGGTCCTCCATCTCAGG
Band lengths:
245-371
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]