DreEX0021730 @ danRer10
Exon Skipping
Gene
ENSDARG00000034307 | chrne
Description
cholinergic receptor, nicotinic, epsilon [Source:ZFIN;Acc:ZDB-GENE-040808-32]
Coordinates
chr5:37843204-37845256:-
Coord C1 exon
chr5:37845056-37845256
Coord A exon
chr5:37843402-37843516
Coord C2 exon
chr5:37843204-37843318
Length
115 bp
Sequences
Splice sites
3' ss Seq
TTACTGTCTCTTCTTCTTAGCTG
3' ss Score
8.76
5' ss Seq
CAAGTCAGT
5' ss Score
2.74
Exon sequences
Seq C1 exon
AAAACGGTGAGTGGGCAATCAAGCACCGGCCCGCCCGAAAGCTGACAAACCAACGCTACTCTCCAGATGACCTGGAGTACCAGGAGGTATATTACAACATCATAATCCAAAGGAAACCGCTTTTCTACGTCATCAACATCATCCTGCCCTGCTCTCTCATCTCCTCTCTGGTCGTTCTTGCCTATTTTCTACCTGCAAAAG
Seq A exon
CTGGCGGGCAGAAGTTGACTGTGTCCATTTCTGTTCTTTTGGCTCAGACTGTGTTTCTCATCCTGATTTCTCAGAAAATACCAGAAACGTCACTGTCTGTGCCTCTTATTGGCAA
Seq C2 exon
GTATTTAATTTTCGTGATGTCTATGACCACACTCATTGTGACCAACTGCATTATTGTGCTCAACTACTCCCTCCGCAGTCCCAGCACACACAACATGTCCCAGAGCATCAAGCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000034307-'6-10,'6-8,8-10
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=PD(18.0=57.4),PF0293211=Neur_chan_memb=PU(26.9=30.9)
A:
PF0293211=Neur_chan_memb=FE(48.7=100)
C2:
PF0293211=Neur_chan_memb=FE(15.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGAAAGCTGACAAACCAACGC
R:
GACTGCGGAGGGAGTAGTTGA
Band lengths:
246-361
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]