Special

MmuEX0011390 @ mm10

Exon Skipping

Gene
ENSMUSG00000014609 | Chrne
Description
cholinergic receptor, nicotinic, epsilon polypeptide [Source:MGI Symbol;Acc:MGI:87894]
Coordinates
chr11:70615661-70617347:-
Coord C1 exon
chr11:70617147-70617347
Coord A exon
chr11:70616955-70617069
Coord C2 exon
chr11:70615661-70615775
Length
115 bp
Sequences
Splice sites
3' ss Seq
TCACAGCTTGCTCCCTCTAGCTG
3' ss Score
5.8
5' ss Seq
CAGGTAATC
5' ss Score
6.99
Exon sequences
Seq C1 exon
AGAATGGAGAATGGGCCATAGACTACTGCCCAGGCATGATTCGCCGCTATGAGGGAGGTTCCACAGAAGGTCCTGGAGAAACTGACGTCATCTATACGCTCATCATCCGCCGGAAGCCGCTTTTTTACGTCATTAACATCATTGTGCCTTGCGTGCTCATTTCTGGCTTGGTGCTGCTCGCTTACTTCCTGCCTGCGCAGG
Seq A exon
CTGGTGGCCAGAAATGCACGGTCTCTATCAACGTCCTGCTAGCCCAGACTGTCTTCTTGTTTCTAATTGCCCAGAAAATTCCAGAGACTTCTCTGAGCGTGCCACTGCTGGGCAG
Seq C2 exon
GTATCTTATATTCGTCATGGTGGTTGCCACGCTCATTGTCATGAATTGCGTCATCGTGCTCAACGTATCTTTGAGGACGCCAACGACTCATGCTACATCCCCTCGGCTGCGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000014609_CASSETTE1
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0293118=Neur_chan_LBD=PD(18.0=57.4),PF0293211=Neur_chan_memb=PU(9.2=30.9)

							
A:
PF0293211=Neur_chan_memb=FE(16.6=100)

							
C2:
PF0293211=Neur_chan_memb=FE(16.6=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000014753





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000099616
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm9




Conservation


Human
(hg38)
chr17:4900793-4900907 
ALTERNATIVE
HsaEX0015331
(CHRNE)
Human
(hg19)
chr17:4804088-4804202 
ALTERNATIVE
HsaEX0015331
(CHRNE)
Rat
(rn6)
chr10:57241027-57241141 
HIGH PSI
RnoEX0021238
(Chrne)
Cow
(bosTau6)
chr19:27120724-27120838 
HIGH PSI
BtaEX6086394
(CHRNE)
Chicken
(galGal4)
chr9:15082859-15082973 
HIGH PSI
GgaEX1015450
(CHRNG)
Chicken
(galGal3)
chr9:16712961-16713075 
HIGH PSI
GgaEX1015450
(F1NJ22_CHICK)
Zebrafish
(danRer10)
chr5:37843402-37843516 
HIGH PSI
DreEX0021730
(chrne)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TGAGGGAGGTTCCACAGAAGG

				
R: 
ATGTAGCATGAGTCGTTGGCG

				
Band lengths: 
250-365

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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