DreEX0023436 @ danRer10
Exon Skipping
Gene
ENSDARG00000101816 | col5a3b
Description
collagen, type V, alpha 3b [Source:ZFIN;Acc:ZDB-GENE-110728-4]
Coordinates
chr1:58548758-58549539:-
Coord C1 exon
chr1:58549260-58549539
Coord A exon
chr1:58548901-58549013
Coord C2 exon
chr1:58548758-58548826
Length
113 bp
Sequences
Splice sites
3' ss Seq
TGTGTGACCTTTGACCTCAGGTG
3' ss Score
8.55
5' ss Seq
ACGGTGAGA
5' ss Score
8.94
Exon sequences
Seq C1 exon
GGCTCTGCAGCGGTGGGCATGGCAGCTCCTCCAGCTCTGGGTAAGCGGCGCAGGCATGTGGAGGTGTCTGTGGACGGAGCGGCGCTGGAGGAAGCCGGCTCACAGGTGGAGCAGCAGATGGAGGAGGTGCAGACGGAGGAGGTGCAGATGGAGGAGGTGTTTGCGTCTCTGGCCTCCATGCGCACTGATGTGGAGGGTTTGAGGACGCCGCTGGGCTCCTTCCACAGCCCCGCGCGCACATGCAGAGAGCTGCGGCTCTGCCACCCGGAGTACCCCGACG
Seq A exon
GTGTGTACTGGATCGACCCTAACCAGGGCTGCCACAGAGACGCCTTTAAAGTGTTCTGCAACTTCACCGCTGACGGAGAGACGTGTCTGCAGCCACACAGCAGCGTGCAGACG
Seq C2 exon
GTGAAAATGGCTTCATGGAGCAAAGAGAAGCCCGGAACCTGGTTCAGCACGTTCAAGAAGGGCTCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000101816-'66-67,'66-66,67-67
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.723 A=0.000 C2=0.043
Domain overlap (PFAM):
C1:
PF0139113=Collagen=PD(15.9=10.6),PF0141013=COLFI=PU(12.7=28.7)
A:
PF0141013=COLFI=FE(17.5=100)
C2:
PF0141013=COLFI=FE(10.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACAGGTGGAGCAGCAGATG
R:
TGTGAGCCCTTCTTGAACGTG
Band lengths:
248-361
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]