DreEX0032427 @ danRer10
Exon Skipping
Gene
ENSDARG00000099139 | fbxw5
Description
F-box and WD repeat domain containing 5 [Source:ZFIN;Acc:ZDB-GENE-040426-2571]
Coordinates
chr5:53821780-53830014:-
Coord C1 exon
chr5:53829857-53830014
Coord A exon
chr5:53826402-53826585
Coord C2 exon
chr5:53821780-53821963
Length
184 bp
Sequences
Splice sites
3' ss Seq
CACATTTGCTGCTTTTACAGCTT
3' ss Score
7.19
5' ss Seq
TGGGTAGAG
5' ss Score
-1.16
Exon sequences
Seq C1 exon
CGGCTGTGTCGTGGTACAGGGAGTTCAAGCGGCTATATGACTGCATCCCCTGTGTAGAGGTTCAGACTTTAAAGGAGCATCATGATCAGGTGCTGCATTTAGCCTTCTCTCACCGCGGCCATCGATTTTCCTCCTGTTCCAAAGACTGCACTGTTAAG
Seq A exon
CTTTGGGACACCGAGCAGTCCGATGGCACCATCTCTCTGGTTCACAGCTGCAGCATGCGACAGTATAACTGGGGCTACACTCAGTTCTCTCAGTTTAACGCTGATGACACACTCCTGCTGGTGTCAGGGGTCTACCTGGGCCCACATCACTCCTCCGCTGGAGAGATTGCAGTCTTCAGTTTGG
Seq C2 exon
GGTTGTCAGAGCATAGCCACTCGTGAGCGAGAGTTAGAGACGAAGGTGGCCAGGCTGATGGGCAGCAGACGCACCAAAGCTCCTGAACTAAATCTGGTGTCTCCTTCAAATGTTGGAGAAGGAGAGGACATGACCTATCTGCTCTTCACCACTGGCAGCCTCACATATTCCCCTCACCAAATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099139_MULTIEX1-2/5=1-5
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.355
Domain overlap (PFAM):
C1:
PF0040027=WD40=PU(89.7=66.0)
A:
PF0040027=WD40=PD(5.1=3.2)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCATCATGATCAGGTGCTGC
R:
GGGAATATGTGAGGCTGCCAG
Band lengths:
255-439
Functional annotations
There are 1 annotated functions for this event
PMID: 21725316
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, protein kinase assay, western blot. ELM ID: ELMI003387; ELM sequence: KDDSLLL; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]