DreEX0045920 @ danRer10
Exon Skipping
Gene
ENSDARG00000062686 | megf11
Description
multiple EGF-like-domains 11 [Source:ZFIN;Acc:ZDB-GENE-060503-252]
Coordinates
chr18:19178784-19183200:-
Coord C1 exon
chr18:19183082-19183200
Coord A exon
chr18:19179172-19179300
Coord C2 exon
chr18:19178784-19178912
Length
129 bp
Sequences
Splice sites
3' ss Seq
AGGATATCTCTTTCCTTCAGGCT
3' ss Score
7.74
5' ss Seq
AGAGTGAGT
5' ss Score
7.36
Exon sequences
Seq C1 exon
TGGAAAATTTGGGAAAGCCTGCGCTGAGATCTGTCTGTGCACTAACAATGGAACCTGCAATCCTATTGATGGCTCTTGCCAATGCTTCCCAGGCTGGACCGGAGACGACTGCTCACAGA
Seq A exon
GCTGTCCCTCAGGGTTTTATGGGCGAGACTGTGCAGAGGTGTGCCGCTGTCAAAATGGAGCTGACTGTGATCATATCACTGGCCAGTGTGCCTGTCGGACTGGCTTTATTGGGACCAACTGTGAACAGA
Seq C2 exon
AGTGTCCTCCCGGGACATTTGGCTATGGCTGTCAGCAGCTGTGTGAATGTATGAACAACGCCACCTGCGACTATGTGACTGGAACCTGCTACTGCAGCCCGGGATACAAAGGCATCCGCTGTGACCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062686_MULTIEX1-2/2=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF079748=EGF_2=WD(100=70.5)
A:
PF0005319=Laminin_EGF=PU(70.2=75.0)
C2:
PF0005319=Laminin_EGF=PD(27.7=29.5),PF0005319=Laminin_EGF=PU(82.9=65.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAAATTTGGGAAAGCCTGCG
R:
GTCACAGCGGATGCCTTTGTA
Band lengths:
243-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]