DreEX0054322 @ danRer10
Exon Skipping
Gene
ENSDARG00000011671 | pde6b
Description
phosphodiesterase 6B, cGMP-specific, rod, beta [Source:ZFIN;Acc:ZDB-GENE-090421-2]
Coordinates
chr21:20334116-20339971:+
Coord C1 exon
chr21:20334116-20334199
Coord A exon
chr21:20334292-20334445
Coord C2 exon
chr21:20337308-20339971
Length
154 bp
Sequences
Splice sites
3' ss Seq
CCTCTCCTTGTGTATCACAGGAG
3' ss Score
8.48
5' ss Seq
ACGGTTTGA
5' ss Score
2.58
Exon sequences
Seq C1 exon
CCCATGATGGACAGAAACAAAGCTGCAGAGCTGCCGAAGCTTCAGTGTGGTTTTATTGACTTCGTCTGTACTTTCGTCTATAAG
Seq A exon
GAGTTCTCCCGTTTCCATCCTGAAATCAAGCCAATGTTTGATGGTATTCTAAACAATCGGATGCATTGGAAAGAGAGGCAGGAGGAATATGAGGCAAAACTAAAGGCAATGGAAGAGGCAGTCAAAGCCAGACAGGAAGCAGCTGCCAAAAACG
Seq C2 exon
CTGCAAATTCCAACAGCACCGGCGGATCAGGGTCCAAGACCTGCTCTATCTGTTAGAGAAAAGACAACAGTACAGAGAGCAAGGCATCTCAAGATGCAGCCTGGCCTCCTAAGTATTAAGTCAAACAGGGGGAGATCATCCTTATCCCTAGGATTTGCAAGGATCTGATCAAAGAGTGAGTGTAAAAAAACATATCAATGCTCTCAGCAAGTCACAAGACCACCTACTTACTAAAACATTGTTGGATCTTGCCTTGCAGTTAGTATTACTCTTCAATTGTCTTTCCTTTTGGTCATAGTGTTTGTTTTAAGCAGTTTCATTGAGCGATAGACGGTTTCAATAATGACGATGCATTTTAAATGTACTACAGAAGTGAATGTTTTTCATTTAGTCCCTTACTGGACATTGCCTGATACTTGATATCAACCAGGTCCACAACAAGTTGATGTACAATCATGTGCAGAATGTTATTGTAATTTAAGGAACTTGTGGTAATAAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011671-'19-22,'19-21,20-22
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref, Alt. Stop)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.154 C2=0.389
Domain overlap (PFAM):
C1:
PF0023314=PDEase_I=FE(10.8=100)
A:
PF0023314=PDEase_I=PD(7.6=36.5),PF141951=DUF4316=PU(85.7=80.8)
C2:
PF141951=DUF4316=PD(12.2=31.6)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACTTCGTCTGTACTTTCGTCT
R:
AGGTGGTCTTGTGACTTGCTG
Band lengths:
253-407
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]