DreEX0060816 @ danRer10
Exon Skipping
Gene
ENSDARG00000015971 | rapgef1b
Description
Rap guanine nucleotide exchange factor (GEF) 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1001]
Coordinates
chr21:3996401-4001079:+
Coord C1 exon
chr21:3996401-3996543
Coord A exon
chr21:4000743-4000823
Coord C2 exon
chr21:4000901-4001079
Length
81 bp
Sequences
Splice sites
3' ss Seq
ATTTTACCTCTTCCCAACAGGGA
3' ss Score
10.26
5' ss Seq
AGGGTTGCC
5' ss Score
-10.15
Exon sequences
Seq C1 exon
AATCCCAGAAGTCTCACCTGTCGTCCTTCACGATGAAACTGATGAAGTTTCATTCCCCGAAGATTAAACGGACCCCATCTAAGAAAGGCAAACAACTCCAGCCTGAGCCTGCAGTGAAAACGCCAGAGAAGCCAGTCAACAAG
Seq A exon
GGAAAACCACCAACAATCAAGAAGAAATGTGGTTATAATGGAAGTCAATGGGGCAAAAAAAGCCACCAACATAACGAAAGG
Seq C2 exon
AAGGTGAGTCGACTGGAGGAACAGGAGAAGGATGTGGTCAGCGCTCTGAGATACTTTAAGACGATTGTGGACAAGATGAACGTTGACACCAAAGTTCTCCAGATGCTTCCTGGTTCGGCTAGTAAAGTTTTAGAGGCTATTTTGCCTCTGATGCAGGTCGAGGCTCGGATACAGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015971-'4-4,'4-3,5-4
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.872 A=1.000 C2=0.104
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
ENSDART00000123759fB5123
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCCAGAAGTCTCACCTGTCG
R:
CTCCTGTTCCTCCAGTCGACT
Band lengths:
168-249
Functional annotations
There are 1 annotated functions for this event
PMID: 22749401
Inclusion enhances interaction with GRB2, and reduces with CRK. By LUMIER.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]